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PDBsum entry 4cln
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Calcium binding protein
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PDB id
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4cln
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References listed in PDB file
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Key reference
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Title
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Structure of a recombinant calmodulin from drosophila melanogaster refined at 2.2-A resolution.
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Authors
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D.A.Taylor,
J.S.Sack,
J.F.Maune,
K.Beckingham,
F.A.Quiocho.
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Ref.
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J Biol Chem, 1991,
266,
21375-21380.
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PubMed id
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Abstract
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The crystal structure of calmodulin (Mr 16,700, 148 residues) from Drosophila
melanogaster as expressed in a bacterial system has been determined and refined
at 2.2-A resolution. Starting with the structure of mammalian calmodulin, we
produced an extensively refitted and refined model with a conventional
crystallographic R value of 0.197 for the 5,239 reflections (F greater than or
equal to 2 sigma (F)) within the 10.0-2.2-A resolution range. The model includes
1,164 protein atoms, 4 calcium ions, and 78 water molecules and has root mean
square deviations from standard values of 0.018 A for bond lengths and 0.043 A
for angle distances. The overall structure is similar to mammalian calmodulin,
with a seven-turn central helix connecting the two calcium-binding domains. The
"dumb-bell" shaped molecule contains seven alpha-helices and four "EF hand"
calcium-binding sites. Although the amino acid sequences of mammalian and
Drosophila calmodulins differ by only three conservative amino acid changes, the
refined model reveals a number of significant differences between the two
structures. Superimposition of the structures yields a root mean square
deviation of 1.22 A for the 1,120 equivalent atoms. The calcium-binding domains
have a root mean square deviation of 0.85 A for the 353 equivalent atoms. There
are also differences in the amino terminus, the bend of the central alpha-helix,
and the orientations of some of the side chains.
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Secondary reference #1
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Title
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Structure and sequence of the drosophila melanogaster calmodulin gene.
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Authors
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V.L.Smith,
K.E.Doyle,
J.F.Maune,
R.P.Munjaal,
K.Beckingham.
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Ref.
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J Mol Biol, 1987,
196,
471-485.
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PubMed id
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