PDBsum entry 4cfg

Ligase

PDB id: 4cfg

Contents

Protein chains

164 a.a.

Waters ×8

PDB id:

4cfg

Name:

Ligase

Title:

Structure of the trim25 coiled-coil

Structure:

E3 ubiquitin/isg15 ligase trim25. Chain: a, b. Synonym: estrogen-responsive finger protein, ring finger protein 147, tripartite motif-containing protein 25, ubiquitin/isg15-conjugating enzyme trim25, zinc finger protein 147, trim. Engineered: yes. Mutation: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

2.80Å R-factor: 0.257 R-free: 0.309

Authors:

L.James

Key ref:

L.James Structure of the trim25 coiled-Coil. To be published, .

Date:

15-Nov-13 Release date: 10-Dec-14

Protein chains

Q14258  (TRI25_HUMAN) -  E3 ubiquitin/ISG15 ligase TRIM25 from Homo sapiens

Seq: 630 a.a.

Struc: 164 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class 2: E.C.2.3.2.27 - RING-type E3 ubiquitin transferase.
• Reaction: S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N₆- ubiquitinyl-[acceptor protein]-L-lysine
• Enzyme class 3: E.C.6.3.2.n3 - ?????

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.