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PDBsum entry 4cbm
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PDB id:
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Hydrolase
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Title:
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Pestivirus ns3 helicase
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Structure:
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Serine protease ns3. Chain: a, b, c, d. Fragment: helicase domain, 1792-2280. Synonym: non-structural protein 3. Ec: 3.4.21.113, 3.6.1.15, 3.6.4.13. Engineered: yes. Mutation: yes
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Source:
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Classical swine fever virus. Organism_taxid: 11096. Strain: alfort. Expressed in: escherichia coli. Expression_system_taxid: 511693.
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Resolution:
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3.27Å
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R-factor:
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0.236
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R-free:
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0.283
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Authors:
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M.A.Tortorici,S.Duquerroy,J.Kwok,C.Vonrhein,J.Perez,B.Lamp, G.Bricogne,T.Rumenapf,P.Vachette,F.A.Rey
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Key ref:
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M.A.Tortorici
et al.
(2015).
X-ray structure of the pestivirus NS3 helicase and its conformation in solution.
J Virol,
89,
4356-4371.
PubMed id:
DOI:
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Date:
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14-Oct-13
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Release date:
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21-Jan-15
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PROCHECK
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Headers
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References
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P19712
(POLG_CSFAT) -
Genome polyprotein from Classical swine fever virus (strain Alfort/Tuebingen)
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Seq:
Struc:
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3898 a.a.
467 a.a.*
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Key: |
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PfamA domain |
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Secondary structure |
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*
PDB and UniProt seqs differ
at 1 residue position (black
cross)
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Enzyme class 1:
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E.C.2.7.7.48
- RNA-directed Rna polymerase.
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Reaction:
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RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate
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RNA(n)
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+
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ribonucleoside 5'-triphosphate
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=
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RNA(n+1)
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+
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diphosphate
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Enzyme class 2:
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E.C.3.4.21.113
- pestivirus NS3 polyprotein peptidase.
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Enzyme class 3:
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E.C.3.4.22.-
- ?????
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Enzyme class 4:
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E.C.3.6.1.15
- nucleoside-triphosphate phosphatase.
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Reaction:
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a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'-diphosphate + phosphate + H+
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ribonucleoside 5'-triphosphate
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+
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H2O
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=
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ribonucleoside 5'-diphosphate
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+
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phosphate
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+
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H(+)
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Enzyme class 5:
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E.C.3.6.4.13
- Rna helicase.
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Reaction:
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ATP + H2O = ADP + phosphate + H+
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ATP
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+
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H2O
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=
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ADP
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+
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phosphate
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+
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H(+)
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Note, where more than one E.C. class is given (as above), each may
correspond to a different protein domain or, in the case of polyprotein
precursors, to a different mature protein.
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Molecule diagrams generated from .mol files obtained from the
KEGG ftp site
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DOI no:
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J Virol
89:4356-4371
(2015)
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PubMed id:
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X-ray structure of the pestivirus NS3 helicase and its conformation in solution.
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M.A.Tortorici,
S.Duquerroy,
J.Kwok,
C.Vonrhein,
J.Perez,
B.Lamp,
G.Bricogne,
T.Rümenapf,
P.Vachette,
F.A.Rey.
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ABSTRACT
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Links
