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UniProt functional annotation for Q6LZY8 | ||
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| UniProt code: Q6LZY8. |
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| Organism: | |
Methanococcus maripaludis (strain S2 / LL). |
| Taxonomy: | |
Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales; Methanococcaceae; Methanococcus. |
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| Function: | |
Endopeptidase which proteolytically removes the C-terminal three residues of farnesylated peptides containing the CAAX motif where C is cysteine, A is an aliphatic amino acid and X is any amino acid. Cleaves the CAAX motif C-terminal to both P1 and P1' positions. Hydrolysis depends on a farnesylated cysteine residue and no activity is shown towards geranylgeranylated peptides. {ECO:0000269|PubMed:24291792}. |
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| Activity regulation: | |
Activity is unaffected by metalloprotease inhibitors 5 mM EDTA and 5 mM Zn(2+). Activity partially inhibited by 1,10-phenanthroline and 1,7-phenanthroline. {ECO:0000269|PubMed:24291792}. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=19.7 uM for peptide substrate DABCYL-ARSGAKASGC(farnesyl)LVS-EDANS where EDANS is 5-[(2-aminoethyl)amino]naphthalene-1-sulphonic acid fluorophore and DABCYL is 4-{[4-(dimethylamino)phenyl]azo}benzoic acid quencher {ECO:0000269|PubMed:24291792}; Note=kcat is 0.175 s(-1) for peptide substrate DABCYL- ARSGAKASGC(farnesyl)LVS-EDANS. {ECO:0000269|PubMed:24291792}; |
| Subcellular location: | |
Cell membrane {ECO:0000269|PubMed:24291792}; Multi-pass membrane protein {ECO:0000269|PubMed:24291792}. |
| Similarity: | |
Belongs to the peptidase U48 family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.