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PDBsum entry 4c56
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Immune system/toxin
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PDB id
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4c56
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Contents |
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192 a.a.
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240 a.a.
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227 a.a.
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178 a.a.
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184 a.a.
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13 a.a.
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198 a.a.
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PDB id:
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| Name: |
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Immune system/toxin
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Title:
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X-ray structure of the complex between staphylococcal enterotoxin b, t cell receptor and major histocompatibility complex class ii
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Structure:
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T cell receptor alpha chain. Chain: a, g. Engineered: yes. T cell receptor beta chain. Chain: b, h. Engineered: yes. Enterotoxin b. Chain: c, i. Fragment: ob-domain and beta-grasp domain, residues 13-250.
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Source:
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Homo sapiens. Human. Organism_taxid: 9606. Cell: t-lymphocyte. Expressed in: escherichia coli. Expression_system_taxid: 469008. Expression_system_variant: star. Staphylococcus aureus. Organism_taxid: 1280.
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Resolution:
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2.90Å
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R-factor:
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0.234
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R-free:
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0.251
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Authors:
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K.E.J.Rodstrom,K.Elbing,K.Lindkvist-Petersson
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Key ref:
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K.E.Rödström
et al.
(2014).
Structure of the superantigen staphylococcal enterotoxin B in complex with TCR and peptide-MHC demonstrates absence of TCR-peptide contacts.
J Immunol,
193,
1998-2004.
PubMed id:
DOI:
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Date:
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10-Sep-13
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Release date:
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23-Jul-14
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PROCHECK
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Headers
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References
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P01848
(TCA_HUMAN) -
T cell receptor alpha chain constant from Homo sapiens
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Seq:
Struc:
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140 a.a.
192 a.a.*
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A0A5B9
(TRBC2_HUMAN) -
T cell receptor beta constant 2 from Homo sapiens
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Seq:
Struc:
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178 a.a.
240 a.a.*
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P01552
(ETXB_STAAU) -
Enterotoxin type B from Staphylococcus aureus
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Seq:
Struc:
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266 a.a.
227 a.a.
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P01903
(DRA_HUMAN) -
HLA class II histocompatibility antigen, DR alpha chain from Homo sapiens
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Seq:
Struc:
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254 a.a.
178 a.a.
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P01911
(2B1F_HUMAN) -
HLA class II histocompatibility antigen, DRB1 beta chain from Homo sapiens
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Seq:
Struc:
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266 a.a.
184 a.a.*
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DOI no:
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J Immunol
193:1998-2004
(2014)
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PubMed id:
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Structure of the superantigen staphylococcal enterotoxin B in complex with TCR and peptide-MHC demonstrates absence of TCR-peptide contacts.
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K.E.Rödström,
K.Elbing,
K.Lindkvist-Petersson.
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ABSTRACT
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Superantigens are immune-stimulatory toxins produced by Staphylococcus aureus,
which are able to interact with host immune receptors to induce a massive
release of cytokines, causing toxic shock syndrome and possibly death. In this
article, we present the x-ray structure of staphylococcal enterotoxin B (SEB) in
complex with its receptors, the TCR and MHC class II, forming a ternary complex.
The structure, in combination with functional analyses, clearly shows how SEB
adopts a wedge-like position when binding to the β-chain of TCR, allowing for
an interaction between the α-chain of TCR and MHC. Furthermore, the binding
mode also circumvents contact between TCR and the peptide presented by MHC,
which enables SEB to initiate a peptide-independent activation of T cells.
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