PDBsum entry 4c0l

Hydrolase

PDB id: 4c0l

Contents

Protein chain

404 a.a.

Ligands

UNX

SO4 ×3

GDP

HSE

Metals

_NA

_MG ×2

Waters ×17

References listed in PDB file

Key reference

• Title: Structural coupling of the ef hand and c-Terminal gtpase domains in the mitochondrial protein miro.
• Authors: J.L.Klosowiak, P.J.Focia, S.Chakravarthy, E.C.Landahl, D.M.Freymann, S.E.Rice.
• Ref.: Embo Rep, 2013, 14, 968-974. [DOI no: 10.1038/embor.2013.151]
• PubMed id: 24071720

Abstract

Miro is a highly conserved calcium-binding GTPase at the regulatory nexus of mitochondrial transport and autophagy. Here we present crystal structures comprising the tandem EF hand and carboxy terminal GTPase (cGTPase) domains of Drosophila Miro. The structures reveal two previously unidentified 'hidden' EF hands, each paired with a canonical EF hand. Each EF hand pair is bound to a helix that structurally mimics an EF hand ligand. A key nucleotide-sensing element and a Pink1 phosphorylation site both lie within an extensive EF hand-cGTPase interface. Our results indicate structural mechanisms for calcium, nucleotide and phosphorylation-dependent regulation of mitochondrial function by Miro.