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PDBsum entry 4bzk
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Protein transport
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PDB id
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4bzk
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Contents |
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691 a.a.
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279 a.a.
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280 a.a.
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References listed in PDB file
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Key reference
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Title
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The structure of the copii transport-Vesicle coat assembled on membranes.
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Authors
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G.Zanetti,
S.Prinz,
S.Daum,
A.Meister,
R.Schekman,
K.Bacia,
J.A.Briggs.
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Ref.
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Elife, 2013,
2,
e00951.
[DOI no: ]
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PubMed id
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Abstract
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Coat protein complex II (COPII) mediates formation of the membrane vesicles that
export newly synthesised proteins from the endoplasmic reticulum. The inner
COPII proteins bind to cargo and membrane, linking them to the outer COPII
components that form a cage around the vesicle. Regulated flexibility in coat
architecture is essential for transport of a variety of differently sized
cargoes, but structural data on the assembled coat has not been available. We
have used cryo-electron tomography and subtomogram averaging to determine the
structure of the complete, membrane-assembled COPII coat. We describe a novel
arrangement of the outer coat and find that the inner coat can assemble into
regular lattices. The data reveal how coat subunits interact with one another
and with the membrane, suggesting how coordinated assembly of inner and outer
coats can mediate and regulate packaging of vesicles ranging from small spheres
to large tubular carriers. DOI:http://dx.doi.org/10.7554/eLife.00951.001.
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