PDBsum entry 4bsz

RNA binding protein

PDB id: 4bsz

Contents

Protein chains

187 a.a.

150 a.a.

PDB id:

4bsz

Name:

RNA binding protein

Title:

Crystal structure of the yeast ribosomal protein rps3 in complex with its chaperone yar1

Structure:

40s ribosomal protein s3. Chain: a. Synonym: rp13, ys3. Engineered: yes. Ankyrin repeat-containing protein yar1. Chain: b. Engineered: yes

Source:

Saccharomyces cerevisiae. Baker's yeast. Organism_taxid: 580240. Strain: w303. Expressed in: escherichia coli. Expression_system_taxid: 469008. Expression_system_variant: ril.

Resolution:

2.84Å R-factor: 0.191 R-free: 0.225

Authors:

S.Holzer,N.Ban,S.Klinge

Key ref:

S.Holzer et al. (2013). Crystal structure of the yeast ribosomal protein rpS3 in complex with its chaperone Yar1. J Mol Biol, 425, 4154-4160. PubMed id: 24021814 DOI: 10.1016/j.jmb.2013.08.022

Date:

12-Jun-13 Release date: 04-Sep-13

Protein chain

P05750  (RS3_YEAST) -  Small ribosomal subunit protein uS3 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 240 a.a.

Struc: 187 a.a.

Protein chain

P46683  (YAR1_YEAST) -  Ankyrin repeat-containing protein YAR1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 200 a.a.

Struc: 150 a.a.

DOI no: 10.1016/j.jmb.2013.08.022

J Mol Biol 425:4154-4160 (2013)

PubMed id: 24021814

Crystal structure of the yeast ribosomal protein rpS3 in complex with its chaperone Yar1.

S.Holzer, N.Ban, S.Klinge.

ABSTRACT

Eukaryotic ribosome assembly involves a plethora of factors, which ensure that a correctly folded ribosome contains all ribosomal protein components. Among these assembly factors, Yar1 has recently emerged as a molecular chaperone for ribosomal protein rpS3 of the small ribosomal subunit (40S) in yeast. In complex with its chaperone, rpS3 is imported into the nucleus and protected from aggregation. How rpS3 and other ribosomal proteins are initially sequestered and subsequently integrated into pre-ribosomal particles is currently poorly understood. Here, we present the crystal structure of yeast rpS3 in complex with its chaperone Yar1 at 2.8Å resolution. The crystal structure rationalizes how Yar1 can protect rpS3 from aggregation while facilitating nuclear import and suggests a mechanism for a stepwise exchange of molecular partners that ribosomal proteins interact with during ribosome assembly.