PDBsum entry 4bqk

Transport protein

PDB id: 4bqk

Contents

Protein chains

424 a.a.

12 a.a.

14 a.a.

Ligands

PEG ×9

Waters ×395

PDB id:

4bqk

Name:

Transport protein

Title:

Rice importin_alpha : vird2nls complex

Structure:

Importin subunit alpha-1a. Chain: a, b. Fragment: residues 73-526. Engineered: yes. T-DNA border endonuclease vird2. Chain: c, d. Fragment: residues 415-434. Synonym: vird2nls. Engineered: yes

Source:

Oryza sativa. Rice. Organism_taxid: 4530. Expressed in: escherichia coli. Expression_system_taxid: 469008. Agrobacterium tumefaciens. Organism_taxid: 358.

Resolution:

2.00Å R-factor: 0.197 R-free: 0.224

Authors:

C.-W.Chang,R.M.Counago,S.J.Williams,B.Kobe

Key ref:

C.W.Chang et al. (2014). Structural basis of interaction of bipartite nuclear localization signal from Agrobacterium VirD2 with rice importin-α. Mol Plant, 7, 1061-1064. PubMed id: 24503158 DOI: 10.1093/mp/ssu014

Date:

31-May-13 Release date: 19-Feb-14

Protein chains

Q71VM4  (IMA1A_ORYSJ) -  Importin subunit alpha-1a from Oryza sativa subsp. japonica

Seq: 526 a.a.

Struc: 424 a.a.*

Protein chain

P18592  (VIRD2_AGRFC) -  T-DNA border endonuclease VirD2 from Agrobacterium fabrum (strain C58 / ATCC 33970)

Seq: 447 a.a.

Struc: 12 a.a.

Protein chain

P18592  (VIRD2_AGRFC) -  T-DNA border endonuclease VirD2 from Agrobacterium fabrum (strain C58 / ATCC 33970)

Seq: 447 a.a.

Struc: 14 a.a.

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class 1: Chains A, B: E.C.?
• Enzyme class 2: Chains C, D: E.C.3.1.-.-

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

DOI no: 10.1093/mp/ssu014

Mol Plant 7:1061-1064 (2014)

PubMed id: 24503158

Structural basis of interaction of bipartite nuclear localization signal from Agrobacterium VirD2 with rice importin-α.

C.W.Chang, S.J.Williams, R.M.Couñago, B.Kobe.

ABSTRACT

No abstract given.