PDBsum entry 4bnr

Hydolase/inhibitor

PDB id: 4bnr

Contents

Protein chains

237 a.a.

59 a.a.

Ligands

SO4 ×2

Metals

_CA ×4

Waters ×473

PDB id:

4bnr

Name:

Hydolase/inhibitor

Title:

Extremely stable complex of crayfish trypsin with bovine trypsin inhibitor

Structure:

Hepatopancreas trypsin. Chain: a, b. Pancreatic trypsin inhibitor. Chain: i, j. Synonym: bovine pancreatic trypsin inhibitor, aprotinin, basic protease inhibitor, bpi, bpti

Source:

Pontastacus leptodactylus. Narrow-fingered crayfish. Organism_taxid: 6717. Organ: stomach. Bos taurus. Cattle. Organism_taxid: 9913. Organ: pancreas

Resolution:

2.00Å R-factor: 0.151 R-free: 0.182

Authors:

T.Molnar,J.Voros,B.Szeder,K.Takats,J.Kardos,G.Katona,L.Graf

Key ref:

T.Molnár et al. (2013). Comparison of complexes formed by a crustacean and a vertebrate trypsin with bovine pancreatic trypsin inhibitor - the key to achieving extreme stability? Febs J, 280, 5750-5763. PubMed id: 24034223 DOI: 10.1111/febs.12491

Date:

17-May-13 Release date: 04-Sep-13

Protein chains

Q52V24  (Q52V24_ASTLP) -  Hepatopancreas trypsin (Fragment) from Astacus leptodactylus

Seq: 237 a.a.

Struc: 237 a.a.*

Protein chains

P00974  (BPT1_BOVIN) -  Pancreatic trypsin inhibitor from Bos taurus

Seq: 100 a.a.

Struc: 59 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: Chains A, B: E.C.3.4.21.4 - trypsin.
• Reaction: Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

DOI no: 10.1111/febs.12491

Febs J 280:5750-5763 (2013)

PubMed id: 24034223

Comparison of complexes formed by a crustacean and a vertebrate trypsin with bovine pancreatic trypsin inhibitor - the key to achieving extreme stability?

T.Molnár, J.Vörös, B.Szeder, K.Takáts, J.Kardos, G.Katona, L.Gráf.

ABSTRACT

No abstract given.