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PDBsum entry 4bjl
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protein Protein-protein interface(s) links
Immunoglobulin PDB id
4bjl

 

 

 

 

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Contents
Protein chains
216 a.a. *
Waters ×264
* Residue conservation analysis
PDB id:
4bjl
Name: Immunoglobulin
Title: Locw, a lambda 1 type light-chain dimer (bence-jones protein) crystallized in distilled water
Structure: Loc - lambda 1 type light-chain dimer. Chain: a, b. Synonym: bence-jones protein. Other_details: crystallized in distilled water
Source: Homo sapiens. Human. Organism_taxid: 9606
Biol. unit: Dimer (from PQS)
Resolution:
2.40Å     R-factor:   0.155    
Authors: M.Schiffer,D.B.Huang
Key ref: D.B.Huang et al. (1996). Three quaternary structures for a single protein. Proc Natl Acad Sci U S A, 93, 7017-7021. PubMed id: 8692936
Date:
26-May-95     Release date:   07-Dec-95    
Supersedes: 2bjl
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
No UniProt id for this chain
Struc: 216 a.a.
Key:    Secondary structure  CATH domain

 

 
Proc Natl Acad Sci U S A 93:7017-7021 (1996)
PubMed id: 8692936  
 
 
Three quaternary structures for a single protein.
D.B.Huang, C.F.Ainsworth, F.J.Stevens, M.Schiffer.
 
  ABSTRACT  
 
The structure of a multisubunit protein (immunoglobulin light chain) was solved in three crystal forms, differing only in the solvent of crystallization. The three structures were obtained at high ionic strength and low pH, high ionic strength and high pH, and low ionic strength and neutral pH. The three resulting "snapshots" of possible structures show that their variable-domain interactions differ, reflecting their stabilities under specific solvent conditions. In the three crystal forms, the variable domains had different rotational and translational relationships, whereas no alteration of the constant domains was found. The critical residues involved in the observed effect of the solvent are tryptophans and histidines located between the two variable domains in the dimeric structure. Tryptophan residues are commonly found in interfaces between proteins and their subunits, and histidines have been implicated in pH-dependent conformation changes. The quaternary structure observed for a multisubunit protein or protein complex in a crystal may be influenced by the interactions of the constituents within the molecule or complex and/or by crystal packing interactions. The comparison of buried surface areas and hydrogen bonds between the domains forming the molecule and between the molecules forming the crystals suggest that, for this system, the interactions within the molecule are most likely the determining factors.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
19162470 A.M.Gronenborn (2009).
Protein acrobatics in pairs--dimerization via domain swapping.
  Curr Opin Struct Biol, 19, 39-49.  
19180449 K.S.Keating, S.C.Flores, M.B.Gerstein, and L.A.Kuhn (2009).
StoneHinge: hinge prediction by network analysis of individual protein structures.
  Protein Sci, 18, 359-371.  
19801545 V.Sharma, W.Heriot, K.Trisler, and V.Smider (2009).
A human germ line antibody light chain with hydrolytic properties associated with multimerization status.
  J Biol Chem, 284, 33079-33087.  
17582169 D.L.Makino, A.H.Henschen-Edman, S.B.Larson, and A.McPherson (2007).
Bence Jones KWR protein structures determined by X-ray crystallography.
  Acta Crystallogr D Biol Crystallogr, 63, 780-792.
PDB codes: 2old 2omb 2omn
  16508097 D.L.Makino, A.H.Henschen-Edman, and A.McPherson (2005).
Four crystal forms of a Bence-Jones protein.
  Acta Crystallogr Sect F Struct Biol Cryst Commun, 61, 79-82.  
15641119 M.Król, I.Roterman, B.Piekarska, L.Konieczny, J.Rybarska, B.Stopa, P.Spólnik, and E.Szneler (2005).
An approach to understand the complexation of supramolecular dye Congo red with immunoglobulin L chain lambda.
  Biopolymers, 77, 155-162.  
15778960 M.Król, I.Roterman, B.Piekarska, L.Konieczny, J.Rybarska, B.Stopa, and P.Spólnik (2005).
Analysis of correlated domain motions in IgG light chain reveals possible mechanisms of immunological signal transduction.
  Proteins, 59, 545-554.  
12943847 L.Cao, L.Langen, and R.A.Sheldon (2003).
Immobilised enzymes: carrier-bound or carrier-free?
  Curr Opin Biotechnol, 14, 387-394.  
12447901 P.A.Ramsland, and W.Farrugia (2002).
Crystal structures of human antibodies: a detailed and unfinished tapestry of immunoglobulin gene products.
  J Mol Recognit, 15, 248-259.  
11598879 B.Piekarska, L.Konieczny, J.Rybarska, B.Stopa, G.Zemanek, E.Szneler, M.Król, M.Nowak, and I.Roterman (2001).
Heat-induced formation of a specific binding site for self-assembled Congo Red in the V domain of immunoglobulin L chain lambda.
  Biopolymers, 59, 446-456.  
11112521 T.C.Mueser, P.H.Rogers, and A.Arnone (2000).
Interface sliding as illustrated by the multiple quaternary structures of liganded hemoglobin.
  Biochemistry, 39, 15353-15364.
PDB codes: 1g08 1g09 1g0a 1g0b
  10524280 P.R.Pokkuluri, A.Solomon, D.T.Weiss, F.J.Stevens, and M.Schiffer (1999).
Tertiary structure of human lambda 6 light chains.
  Amyloid, 6, 165-171.
PDB codes: 1cd0 2cd0
9739086 P.R.Pokkuluri, D.B.Huang, R.Raffen, X.Cai, G.Johnson, P.W.Stevens, F.J.Stevens, and M.Schiffer (1998).
A domain flip as a result of a single amino-acid substitution.
  Structure, 6, 1067-1073.
PDB codes: 2lve 3lve 4lve
9753478 S.Kim, and B.A.Barry (1998).
Vibrational spectrum associated with the reduction of tyrosyl radical D* in photosystem II: a comparative biochemical and kinetic study.
  Biochemistry, 37, 13882-13892.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.

 

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