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PDBsum entry 4bh6
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Contents |
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(+ 2 more)
304 a.a.
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65 a.a.
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63 a.a.
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65 a.a.
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52 a.a.
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21 a.a.
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20 a.a.
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References listed in PDB file
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Key reference
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Title
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Insights into degron recognition by apc/c coactivators from the structure of an acm1-Cdh1 complex.
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Authors
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J.He,
W.C.Chao,
Z.Zhang,
J.Yang,
N.Cronin,
D.Barford.
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Ref.
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Mol Cell, 2013,
50,
649-660.
[DOI no: ]
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PubMed id
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Abstract
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The anaphase-promoting complex/cyclosome (APC/C) regulates sister chromatid
segregation and the exit from mitosis. Selection of most APC/C substrates is
controlled by coactivator subunits (either Cdc20 or Cdh1) that interact with
substrate destruction motifs-predominantly the destruction (D) box and KEN box
degrons. How coactivators recognize D box degrons and how this is inhibited by
APC/C regulatory proteins is not defined at the atomic level. Here, from the
crystal structure of S. cerevisiae Cdh1 in complex with its specific inhibitor
Acm1, which incorporates D and KEN box pseudosubstrate motifs, we describe the
molecular basis for D box recognition. Additional interactions between Acm1 and
Cdh1 identify a third protein-binding site on Cdh1 that is likely to confer
coactivator-specific protein functions including substrate association. We
provide a structural rationalization for D box and KEN box recognition by
coactivators and demonstrate that many noncanonical APC/C degrons bind APC/C
coactivators at the D box coreceptor.
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