PDBsum entry 4bh6

Cell cycle

PDB id: 4bh6

Contents

Protein chains

(+ 2 more) 304 a.a.

65 a.a.

63 a.a.

65 a.a.

52 a.a.

21 a.a.

20 a.a.

Waters ×28

PDB id:

4bh6

Name:

Cell cycle

Title:

Insights into degron recognition by apc coactivators from the structure of an acm1-cdh1 complex

Structure:

Apc/c activator protein cdh1. Chain: a, b, c, d, e, f, g, h. Fragment: wd40 domain, residues 241-548. Synonym: cdh1, cdc20 homolog 1, homolog of cdc twenty 1. Engineered: yes. Apc/c-cdh1 modulator 1. Chain: i, j, k, l, m, n, o, p. Fragment: residues 59-128. Engineered: yes.

Source:

Saccharomyces cerevisiae. Baker's yeast. Organism_taxid: 4932. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Expression_system_cell_line: sf9.

Resolution:

2.90Å R-factor: 0.221 R-free: 0.257

Authors:

J.He,W.C.H.Chao,Z.Zhang,J.Yang,N.Cronin,D.Barford

Key ref:

J.He et al. (2013). Insights into degron recognition by APC/C coactivators from the structure of an Acm1-Cdh1 complex. Mol Cell, 50, 649-660. PubMed id: 23707760 DOI: 10.1016/j.molcel.2013.04.024

Date:

29-Mar-13 Release date: 05-Jun-13

Protein chains

P53197  (CDH1_YEAST) -  APC/C activator protein CDH1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 566 a.a.

Struc: 304 a.a.

Protein chain

Q08981  (ACM1_YEAST) -  APC/C-CDH1 modulator 1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 209 a.a.

Struc: 65 a.a.

Protein chain

Q08981  (ACM1_YEAST) -  APC/C-CDH1 modulator 1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 209 a.a.

Struc: 63 a.a.

Protein chain

Q08981  (ACM1_YEAST) -  APC/C-CDH1 modulator 1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 209 a.a.

Struc: 65 a.a.

Protein chain

Q08981  (ACM1_YEAST) -  APC/C-CDH1 modulator 1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 209 a.a.

Struc: 52 a.a.

Protein chains

Q08981  (ACM1_YEAST) -  APC/C-CDH1 modulator 1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 209 a.a.

Struc: 21 a.a.

Protein chains

Q08981  (ACM1_YEAST) -  APC/C-CDH1 modulator 1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 209 a.a.

Struc: 20 a.a.

DOI no: 10.1016/j.molcel.2013.04.024

Mol Cell 50:649-660 (2013)

PubMed id: 23707760

Insights into degron recognition by APC/C coactivators from the structure of an Acm1-Cdh1 complex.

J.He, W.C.Chao, Z.Zhang, J.Yang, N.Cronin, D.Barford.

ABSTRACT

The anaphase-promoting complex/cyclosome (APC/C) regulates sister chromatid segregation and the exit from mitosis. Selection of most APC/C substrates is controlled by coactivator subunits (either Cdc20 or Cdh1) that interact with substrate destruction motifs-predominantly the destruction (D) box and KEN box degrons. How coactivators recognize D box degrons and how this is inhibited by APC/C regulatory proteins is not defined at the atomic level. Here, from the crystal structure of S. cerevisiae Cdh1 in complex with its specific inhibitor Acm1, which incorporates D and KEN box pseudosubstrate motifs, we describe the molecular basis for D box recognition. Additional interactions between Acm1 and Cdh1 identify a third protein-binding site on Cdh1 that is likely to confer coactivator-specific protein functions including substrate association. We provide a structural rationalization for D box and KEN box recognition by coactivators and demonstrate that many noncanonical APC/C degrons bind APC/C coactivators at the D box coreceptor.