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UniProt functional annotation for P10486 | ||
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| UniProt code: P10486. |
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| Organism: | |
Escherichia coli. |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. |
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| Function: | |
The EcoR124/3 I enzyme recognizes 5'-GAAN(7)RTCG-3'. Subunit R is required for both nuclease and ATPase activities, but not for modification. |
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| Catalytic activity: | |
Reaction=Endonucleolytic cleavage of DNA to give random double-stranded fragments with terminal 5'-phosphates, ATP is simultaneously hydrolyzed.; EC=3.1.21.3; |
| Subunit: | |
The type I restriction/modification system is composed of three polypeptides R, M and S. |
| Subunit: | |
(Microbial infection) Interacts with Escherichia phage T7 protein Ocr; this interaction leads to the inhibition of the type I bifunctional endonuclease and methyltransferase restriction enzyme R.EcoKI composed of R(2)M(2)S(1). {ECO:0000269|PubMed:32483229}. |
| Miscellaneous: | |
Type I restriction and modification enzymes are complex, multifunctional systems which require ATP, S-adenosyl methionine and magnesium as cofactors and, in addition to their endonucleolytic and methylase activities, are potent DNA-dependent ATPases. |
| Similarity: | |
Belongs to the HsdR family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.