PDBsum entry 4b8e

Ligase

PDB id: 4b8e

Contents

Protein chains

194 a.a.

Ligands

EDO

Metals

_CL ×6

Waters ×224

PDB id:

4b8e

Name:

Ligase

Title:

Pry-spry domain of trim25

Structure:

E3 ubiquitin/isg15 ligase trim25. Chain: a, b. Fragment: pryspry / b30.2, residues 440-634. Synonym: estrogen-responsive finger protein, tripartite motif- containing protein 25, ubiquitin/isg15-conjugating enzyme trim25, zinc finger protein 147. Engineered: yes

Source:

Mus musculus. House mouse. Organism_taxid: 10090. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

1.78Å R-factor: 0.174 R-free: 0.194

Authors:

N.J.Kershaw,A.A.D'Cruz,N.A.Nicola,S.E.Nicholson,J.J.Babon

Key ref:

A.A.D'Cruz et al. (2013). Crystal structure of the TRIM25 B30.2 (PRYSPRY) domain: a key component of antiviral signalling. Biochem J, 456, 231-240. PubMed id: 24015671 DOI: 10.1042/BJ20121425

Date:

27-Aug-12 Release date: 04-Sep-13

Protein chains

Q61510  (TRI25_MOUSE) -  E3 ubiquitin/ISG15 ligase TRIM25 from Mus musculus

Seq: 634 a.a.

Struc: 194 a.a.

Enzyme reactions

• Enzyme class 2: E.C.2.3.2.27 - RING-type E3 ubiquitin transferase.
• Reaction: S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N₆- ubiquitinyl-[acceptor protein]-L-lysine
• Enzyme class 3: E.C.6.3.2.n3 - ?????

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

DOI no: 10.1042/BJ20121425

Biochem J 456:231-240 (2013)

PubMed id: 24015671

Crystal structure of the TRIM25 B30.2 (PRYSPRY) domain: a key component of antiviral signalling.

A.A.D'Cruz, N.J.Kershaw, J.J.Chiang, M.K.Wang, N.A.Nicola, J.J.Babon, M.U.Gack, S.E.Nicholson.

ABSTRACT

No abstract given.