|
|
||
|
|
|
|
|
UniProt functional annotation for P31384 | ||
|
|
|
|
|
|
||
| UniProt code: P31384. |
|
||
| Organism: | |
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). |
| Taxonomy: | |
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces. |
|
||
|
||
| Function: | |
Acts as catalytic component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. CCR4 has 3'-5' RNase activity with a strong preference for polyadenylated substrates and also low exonuclease activity towards single-stranded DNA. Discovered because of its role in the control of ADH2 gene expression. It is required for the expression of genes involved in non-fermentative growth and it mediates or is required for the action of the SPT6 and SPT10 genes. {ECO:0000269|PubMed:11239395, ECO:0000269|PubMed:11889047, ECO:0000269|PubMed:11889048, ECO:0000269|PubMed:9463387}. |
|
||
| Catalytic activity: | |
Reaction=Exonucleolytic cleavage of poly(A) to 5'-AMP.; EC=3.1.13.4; |
| Cofactor: | |
Name=Mg(2+); Xref=ChEBI:CHEBI:18420; |
| Subunit: | |
Subunit of the 1.0 MDa CCR4-NOT core complex that contains CCR4, CAF1, NOT1, NOT2, NOT3, NOT4, NOT5, CAF40 and CAF130. In the complex interacts with NOT1. The core complex probably is part of a less characterized 1.9 MDa CCR4-NOT complex. {ECO:0000269|PubMed:10490603, ECO:0000269|PubMed:11733989, ECO:0000269|PubMed:9463387}. |
| Subcellular location: | |
Cytoplasm {ECO:0000269|PubMed:11239395}. Nucleus {ECO:0000269|PubMed:11239395}. |
| Domain: | |
The 169 C-terminal residues are important for deadenylase activity. |
| Miscellaneous: | |
Present with 2780 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}. |
| Similarity: | |
Belongs to the CCR4/nocturin family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.