PDBsum entry 4ay5

Transferase/peptide

PDB id: 4ay5

Contents

Protein chains

698 a.a.

11 a.a.

Ligands

UDP ×4

NAG ×4

PDB id:

4ay5

Name:

Transferase/peptide

Title:

Human o-glcnac transferase (ogt) in complex with udp and glycopeptide

Structure:

Udp-n-acetylglucosamine--peptide n-acetylglucosaminyl transferase 110 kda subunit. Chain: a, b, c, d. Fragment: tpr (truncated) and catalytic domain, residues 313-1031. Synonym: o-glcnac transferase subunit p110, o-linked n-acetyl glucosamine transferase 110 kda subunit, ogt. Engineered: yes. Gtab1tide. Chain: i, j, k, l.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 511693. Expression_system_variant: arcticexpress (ril). Synthetic: yes. Synthetic construct. Organism_taxid: 32630

Resolution:

3.15Å R-factor: 0.174 R-free: 0.204

Authors:

M.Schimpl,X.Zheng,D.E.Blair,A.W.Schuettelkopf,I.Navratilova, T.Aristotelous,A.T.Ferenbach,M.A.Macnaughtan,V.S.Borodkin,D.M.F.Van Aalten

Key ref:

M.Schimpl et al. (2012). O-GlcNAc transferase invokes nucleotide sugar pyrophosphate participation in catalysis. Nat Chem Biol, 8, 969-974. PubMed id: 23103942

Date:

18-Jun-12 Release date: 24-Oct-12

Protein chains

O15294  (OGT1_HUMAN) -  UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit from Homo sapiens

Seq: 1046 a.a.

Struc: 698 a.a.*

Protein chains

Q15750  (TAB1_HUMAN) -  TGF-beta-activated kinase 1 and MAP3K7-binding protein 1 from Homo sapiens

Seq: 504 a.a.

Struc: 11 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: Chains A, B, C, D: E.C.2.4.1.255 - protein O-GlcNAc transferase.
• Reaction:
  1. L-seryl-[protein] + UDP-N-acetyl-alpha-D-glucosamine = 3-O-(N-acetyl- beta-D-glucosaminyl)-L-seryl-[protein] + UDP + H⁺
  2. L-threonyl-[protein] + UDP-N-acetyl-alpha-D-glucosamine = 3-O- (N-acetyl-beta-D-glucosaminyl)-L-threonyl-[protein] + UDP + H⁺

L-seryl-[protein] + UDP-N-acetyl-alpha-D-glucosamine (Bound ligand (Het Group name = NAG) matches with 47.06% similarity) = 3-O-(N-acetyl- beta-D-glucosaminyl)-L-seryl-[protein] (Bound ligand (Het Group name = UDP) corresponds exactly) + UDP + H(+)

L-threonyl-[protein] + UDP-N-acetyl-alpha-D-glucosamine = 3-O- (N-acetyl-beta-D-glucosaminyl)-L-threonyl-[protein] (Bound ligand (Het Group name = UDP) corresponds exactly) + UDP + H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

Nat Chem Biol 8:969-974 (2012)

PubMed id: 23103942

O-GlcNAc transferase invokes nucleotide sugar pyrophosphate participation in catalysis.

M.Schimpl, X.Zheng, V.S.Borodkin, D.E.Blair, A.T.Ferenbach, A.W.Schüttelkopf, I.Navratilova, T.Aristotelous, O.Albarbarawi, D.A.Robinson, M.A.Macnaughtan, D.M.van Aalten.

ABSTRACT

No abstract given.