PDBsum entry 4asu

Hydrolase

PDB id: 4asu

Contents

Protein chains

487 a.a.

467 a.a.

181 a.a.

83 a.a.

23 a.a.

Ligands

ADP ×6

Metals

_MG ×5

Waters ×308

PDB id:

4asu

Name:

Hydrolase

Title:

F1-atpase in which all three catalytic sites contain bound nucleotide, with magnesium ion released in the empty site

Structure:

Atp synthase subunit alpha, mitochondrial. Chain: a, b, c. Atp synthase subunit beta, mitochondrial. Chain: d, e, f. Atp synthase subunit gamma, mitochondrial. Chain: g. Synonym: f-atpase gamma subunit. Atp synthase subunit delta, mitochondrial. Chain: h.

Source:

Bos taurus. Cattle. Organism_taxid: 9913. Organ: heart. Tissue: muscle. Tissue: muscle

Resolution:

2.60Å R-factor: 0.247 R-free: 0.289

Authors:

D.M.Rees,M.G.Montgomery,A.G.W.Leslie,J.E.Walker

Key ref:

D.M.Rees et al. (2012). Structural evidence of a new catalytic intermediate in the pathway of ATP hydrolysis by F1-ATPase from bovine heart mitochondria. Proc Natl Acad Sci U S A, 109, 11139-11143. PubMed id: 22733764

Date:

03-May-12 Release date: 27-Jun-12

Protein chains

P19483  (ATPA_BOVIN) -  ATP synthase subunit alpha, mitochondrial from Bos taurus

Seq: 553 a.a.

Struc: 487 a.a.*

Protein chains

P00829  (ATPB_BOVIN) -  ATP synthase subunit beta, mitochondrial from Bos taurus

Seq: 528 a.a.

Struc: 467 a.a.

Protein chain

P05631  (ATPG_BOVIN) -  ATP synthase subunit gamma, mitochondrial from Bos taurus

Seq: 298 a.a.

Struc: 181 a.a.

Protein chain

P05630  (ATPD_BOVIN) -  ATP synthase subunit delta, mitochondrial from Bos taurus

Seq: 168 a.a.

Struc: 83 a.a.

Protein chain

P05632  (ATP5E_BOVIN) -  ATP synthase subunit epsilon, mitochondrial from Bos taurus

Seq: 51 a.a.

Struc: 23 a.a.*

* PDB and UniProt seqs differ at 7 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chains D, E, F: E.C.7.1.2.2 - H(+)-transporting two-sector ATPase.
• Reaction: ATP + H2O + 4 H⁺(in) = ADP + phosphate + 5 H⁺(out)

ATP + H2O + 4 × H(+)(in) = ADP (Bound ligand (Het Group name = ADP) corresponds exactly) + phosphate + 5 × H(+)(out)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

Proc Natl Acad Sci U S A 109:11139-11143 (2012)

PubMed id: 22733764

Structural evidence of a new catalytic intermediate in the pathway of ATP hydrolysis by F1-ATPase from bovine heart mitochondria.

D.M.Rees, M.G.Montgomery, A.G.Leslie, J.E.Walker.

ABSTRACT

No abstract given.