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UniProt functional annotation for Q10714 | ||
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| UniProt code: Q10714. |
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| Organism: | |
Drosophila melanogaster (Fruit fly). |
| Taxonomy: | |
Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora. |
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| Function: | |
May be involved in the specific maturation or degradation of a number of bioactive peptides. May play a role in the contractions of the heart, gut and testes, and in spermatid differentiation. {ECO:0000269|PubMed:12591244}. |
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| Catalytic activity: | |
Reaction=Release of a C-terminal dipeptide, oligopeptide-|-Xaa-Yaa, when Xaa is not Pro, and Yaa is neither Asp nor Glu. Thus, conversion of angiotensin I to angiotensin II, with increase in vasoconstrictor activity, but no action on angiotensin II.; EC=3.4.15.1; Evidence={ECO:0000269|PubMed:7775412}; |
| Cofactor: | |
Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=Binds 1 zinc ion per subunit.; |
| Activity regulation: | |
Inhibited by captopril and, to a lesser extent, by lisinopril, trandolaprilat, fosinoprilat and enalaprilat. {ECO:0000269|PubMed:7775412, ECO:0000269|PubMed:8761461}. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=33.5 uM for angiotensin I {ECO:0000269|PubMed:7775412, ECO:0000269|PubMed:8761461, ECO:0000269|PubMed:9839949}; KM=53.4 uM for N-acetyl-Ser-Asp-Lys-Pro {ECO:0000269|PubMed:7775412, ECO:0000269|PubMed:8761461, ECO:0000269|PubMed:9839949}; KM=2.59 mM for Hip-His-Leu {ECO:0000269|PubMed:7775412, ECO:0000269|PubMed:8761461, ECO:0000269|PubMed:9839949}; KM=10.26 mM for Hip-His-Leu-NH(2) {ECO:0000269|PubMed:7775412, ECO:0000269|PubMed:8761461, ECO:0000269|PubMed:9839949}; KM=372 uM for (Leu5)enkephalin {ECO:0000269|PubMed:7775412, ECO:0000269|PubMed:8761461, ECO:0000269|PubMed:9839949}; KM=1.88 mM for (Leu5)enkephalinamide {ECO:0000269|PubMed:7775412, ECO:0000269|PubMed:8761461, ECO:0000269|PubMed:9839949}; |
| Subcellular location: | |
Secreted, extracellular space. |
| Tissue specificity: | |
Expressed in vesicular structures in spermatocytes and early spermatids (at protein level). {ECO:0000269|PubMed:12591244}. |
| Developmental stage: | |
Expressed in the amnioserosa during germ band elongation, shortening and heart morphogenesis. Expressed in midgut throughout embryogenesis. |
| Ptm: | |
Glycosylated. {ECO:0000269|PubMed:8761461}. |
| Disruption phenotype: | |
Male flies are sterile. {ECO:0000269|PubMed:12591244}. |
| Similarity: | |
Belongs to the peptidase M2 family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.