PDBsum entry 4am3

Transferase/RNA

PDB id: 4am3

Contents

Protein chains

622 a.a.

DNA/RNA

Ligands

__U ×2

_DA

PO4 ×6

Waters ×54

PDB id:

4am3

Name:

Transferase/RNA

Title:

Crystal structure of c. Crescentus pnpase bound to RNA

Structure:

Polyribonucleotide nucleotidyltransferase. Chain: a, b, c. Synonym: polynucleotide phosphorylase, pnpase. Engineered: yes. RNA, 5'-r( Up Ap Ap Cp Up Up Up Gp Gp)-3'. Chain: d, e, h, i. Fragment: co-purified RNA from e. Coli expression strain

Source:

Caulobacter vibrioides. Organism_taxid: 190650. Strain: cb15. Atcc: 19089. Expressed in: escherichia coli. Expression_system_taxid: 469008. Escherichia coli. Organism_taxid: 469008. Strain: bl21(de3)

Resolution:

3.00Å R-factor: 0.212 R-free: 0.254

Authors:

S.W.Hardwick,T.Gubbey,I.Hug,U.Jenal,B.F.Luisi

Key ref:

S.W.Hardwick et al. (2012). Crystal structure of Caulobacter crescentus polynucleotide phosphorylase reveals a mechanism of RNA substrate channelling and RNA degradosome assembly. Open Biol, 2, 120028. PubMed id: 22724061

Date:

07-Mar-12 Release date: 18-Apr-12

Protein chains

Q9AC32  (PNP_CAUVC) -  Polyribonucleotide nucleotidyltransferase from Caulobacter vibrioides (strain ATCC 19089 / CB15)

Seq: 712 a.a.

Struc: 622 a.a.

DNA/RNA chain

U-A-A-C-U-U-U-G-G 9 bases

Enzyme reactions

• Enzyme class: E.C.2.7.7.8 - polyribonucleotide nucleotidyltransferase.
• Reaction: RNA(n+1) + phosphate = RNA(n) + a ribonucleoside 5'-diphosphate

RNA(n+1) (Bound ligand (Het Group name = PO4) corresponds exactly) + phosphate = RNA(n) + ribonucleoside 5'-diphosphate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

Open Biol 2:120028 (2012)

PubMed id: 22724061

Crystal structure of Caulobacter crescentus polynucleotide phosphorylase reveals a mechanism of RNA substrate channelling and RNA degradosome assembly.

S.W.Hardwick, T.Gubbey, I.Hug, U.Jenal, B.F.Luisi.

ABSTRACT

No abstract given.