PDBsum entry 4aez

Cell cycle

PDB id

4aez

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Contents

			Protein chains

					326 a.a.


					180 a.a.


					215 a.a.


					196 a.a.


					185 a.a.

			Waters ×318

PDB id:

4aez

Links

Name:

Cell cycle

Title:

Crystal structure of mitotic checkpoint complex

Structure:

Wd repeat-containing protein slp1. Chain: a, d, g. Fragment: residues 88-488. Synonym: cdc20. Engineered: yes. Mitotic spindle checkpoint component mad2. Chain: b, e, h. Synonym: mad2. Engineered: yes.

Source:

Schizosaccharomyces pombe. Fission yeast. Organism_taxid: 4896. Expressed in: trichoplusia ni. Expression_system_taxid: 7111. Expression_system_cell_line: high five.

Resolution:

2.30Å

R-factor:

0.222

R-free:

0.267

Authors:

K.A.Kulkarni,W.C.H.Chao,Z.Zhang,D.Barford

Key ref:

W.C.Chao et al. (2012). Structure of the mitotic checkpoint complex. Nature, 484, 208-213. PubMed id: 22437499

Date:

13-Jan-12

Release date:

21-Mar-12

PROCHECK

	Headers

	References

Protein chains

P78972 (SLP1_SCHPO) - WD repeat-containing protein slp1 from Schizosaccharomyces pombe (strain 972 / ATCC 24843)

Seq: Struc:					488 a.a. 326 a.a.

Protein chains

O14417 (MAD2_SCHPO) - Mitotic spindle checkpoint component mad2 from Schizosaccharomyces pombe (strain 972 / ATCC 24843)

Seq: Struc:					203 a.a. 180 a.a.*

Protein chain

O59767 (MAD3_SCHPO) - Mitotic spindle checkpoint component mad3 from Schizosaccharomyces pombe (strain 972 / ATCC 24843)

Seq: Struc:					310 a.a. 215 a.a.

Protein chains

O59767 (MAD3_SCHPO) - Mitotic spindle checkpoint component mad3 from Schizosaccharomyces pombe (strain 972 / ATCC 24843)

Seq: Struc:					310 a.a. 196 a.a.

Protein chain

O14417 (MAD2_SCHPO) - Mitotic spindle checkpoint component mad2 from Schizosaccharomyces pombe (strain 972 / ATCC 24843)

Seq: Struc:					203 a.a. 185 a.a.*

Key:

PfamA domain

Secondary structure

CATH domain

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

	Nature 484:208-213 (2012)
PubMed id: 22437499

Structure of the mitotic checkpoint complex.
W.C.Chao, K.Kulkarni, Z.Zhang, E.H.Kong, D.Barford.

ABSTRACT

No abstract given.

Literature references that cite this PDB file's key reference

PubMed id

Reference

23132380

A.Musacchio, and A.Ciliberto (2012).
The spindle-assembly checkpoint and the beauty of self-destruction.

Nat Struct Mol Biol, 19, 1059-1061.

23258294

E.A.Foley, and T.M.Kapoor (2012).
Microtubule attachment and spindle assembly checkpoint signalling at the kinetochore.

Nat Rev Mol Cell Biol, 14, 25-37.

23007861

K.Uzunova, B.T.Dye, H.Schutz, R.Ladurner, G.Petzold, Y.Toyoda, M.A.Jarvis, N.G.Brown, I.Poser, M.Novatchkova, K.Mechtler, A.A.Hyman, H.Stark, B.A.Schulman, and J.M.Peters (2012).
APC15 mediates CDC20 autoubiquitylation by APC/C(MCC) and disassembly of the mitotic checkpoint complex.

Nat Struct Mol Biol, 19, 1116-1123.

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time.