PDBsum entry 4ae6

Transferase

PDB id: 4ae6

Contents

Protein chain

321 a.a.

Ligands

ACT

Waters ×255

PDB id:

4ae6

Name:

Transferase

Title:

Structure and function of the human sperm-specific isoform of protein kinase a (pka) catalytic subunit calpha 2

Structure:

Camp-dependent protein kinase catalytic subunit alpha 2. Chain: a, b. Synonym: pka c-alpha, pka c-alpha 2. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Cell: spermatozoa. Expressed in: escherichia coli. Expression_system_taxid: 511693. Expression_system_variant: rosetta plyss.

Resolution:

2.10Å R-factor: 0.219 R-free: 0.283

Authors:

T.H.Hereng,P.H.Backe,J.Kahmann,C.Scheich,M.Bjoras,B.S.Skalhegg, K.R.Rosendal

Key ref:

T.H.Hereng et al. (2012). Structure and function of the human sperm-specific isoform of protein kinase A (PKA) catalytic subunit Cα2. J Struct Biol, 178, 300-310. PubMed id: 22504716

Date:

09-Jan-12 Release date: 18-Apr-12

Protein chains

P17612  (KAPCA_HUMAN) -  cAMP-dependent protein kinase catalytic subunit alpha from Homo sapiens

Seq: 351 a.a.

Struc: 321 a.a.*

* PDB and UniProt seqs differ at 4 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.2.7.11.11 - cAMP-dependent protein kinase.
• Reaction:
  1. L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H⁺
  2. L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

J Struct Biol 178:300-310 (2012)

PubMed id: 22504716

Structure and function of the human sperm-specific isoform of protein kinase A (PKA) catalytic subunit Cα2.

T.H.Hereng, P.H.Backe, J.Kahmann, C.Scheich, M.Bjørås, B.S.Skålhegg, K.R.Rosendal.

ABSTRACT

No abstract given.