PDBsum entry 4a0b

DNA binding protein/DNA

PDB id: 4a0b

Contents

Protein chains

1090 a.a.

354 a.a.

DNA/RNA

PDB id:

4a0b

Name:

DNA binding protein/DNA

Title:

Structure of hsddb1-drddb2 bound to a 16 bp cpd-duplex (pyrimidine at d-1 position) at 3.8 a resolution (cpd 4)

Structure:

DNA damage-binding protein 1. Chain: a, c. Engineered: yes. Mutation: yes. DNA damage-binding protein 2. Chain: b, d. Fragment: residues 60-423. Synonym: damage-specific DNA-binding protein 2. Engineered: yes.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Expressed in: trichoplusia ni. Expression_system_taxid: 7111. Expression_system_cell_line: high five. Danio rerio. Zebrafish. Organism_taxid: 7955.

Resolution:

3.80Å R-factor: 0.247 R-free: 0.319

Authors:

A.Scrima,E.S.Fischer,S.Iwai,H.Gut,N.H.Thoma

Key ref:

E.S.Fischer et al. (2011). The molecular basis of CRL4DDB2/CSA ubiquitin ligase architecture, targeting, and activation. Cell, 147, 1024-1039. PubMed id: 22118460

Date:

08-Sep-11 Release date: 30-Nov-11

Protein chains

Q16531  (DDB1_HUMAN) -  DNA damage-binding protein 1 from Homo sapiens

Seq: 1140 a.a.

Struc: 1090 a.a.*

Protein chains

Q2YDS1  (DDB2_DANRE) -  DNA damage-binding protein 2 from Danio rerio

Seq: 496 a.a.

Struc: 354 a.a.*

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

DNA/RNA chains

G-G-T-G-A-A-T-TTD-A-G-C-A-G-G 14 bases

C-T-G-C-T-C-C-A-T-T-C-A-C-C 14 bases

G-G-T-G-A-A-T-TTD-A-G-C-A-G 13 bases

C-T-G-C-T-C-C-A-T-T-C-A-C 13 bases

Cell 147:1024-1039 (2011)

PubMed id: 22118460

The molecular basis of CRL4DDB2/CSA ubiquitin ligase architecture, targeting, and activation.

E.S.Fischer, A.Scrima, K.Böhm, S.Matsumoto, G.M.Lingaraju, M.Faty, T.Yasuda, S.Cavadini, M.Wakasugi, F.Hanaoka, S.Iwai, H.Gut, K.Sugasawa, N.H.Thomä.

ABSTRACT

No abstract given.