UniProt functional annotation for Q2YDS1



	UniProt functional annotation for Q2YDS1

UniProt code: Q2YDS1.

Organism: Danio rerio (Zebrafish) (Brachydanio rerio).

Taxonomy: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes; Danionidae; Danioninae; Danio.

Function: Protein, which is both involved in DNA repair and protein ubiquitination, as part of the UV-DDB complex and DCX (DDB1-CUL4-X-box) complexes, respectively. Core component of the UV-DDB complex (UV- damaged DNA-binding protein complex), a complex that recognizes UV- induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6- 4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also functions as the substrate recognition module for the DCX (DDB2-CUL4-X- box) E3 ubiquitin-protein ligase complex DDB2-CUL4-ROC1 (also known as CUL4-DDB-ROC1 and CUL4-DDB-RBX1). The DDB2-CUL4-ROC1 complex may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV- induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. {ECO:0000250|UniProtKB:Q92466}.

Pathway: Protein modification; protein ubiquitination.

Subunit: Component of the UV-DDB complex which includes ddb1 and ddb2 (PubMed:19109893). Component of a DCX (DDB1-CUL4-X-box) E3 ubiquitin- protein ligase complex that includes cul4a, or cul4b, ddb1, ddb2 and rbx1 (PubMed:22118460). A large number of other DCX complexes may also exist in which an alternate substrate targeting subunit replaces ddb2. These targeting subunits are generally known as DCAF (ddb1- and cul4- associated factor) or CDW (cul4-ddb1-associated WD40-repeat) proteins (By similarity). {ECO:0000250, ECO:0000269|PubMed:19109893, ECO:0000269|PubMed:22118460}.

Subcellular location: Nucleus {ECO:0000250|UniProtKB:Q92466}. Note=Accumulates at sites of DNA damage following UV irradiation. {ECO:0000250|UniProtKB:Q92466}.

Domain: The DWD box is required for interaction with ddb1. {ECO:0000250}.

Domain: Interblade loops of the WD repeat region mediate most of the interaction with DNA. A hairpin between blades 5 and 6 inserts into DNA minor groove and mediates recognition of lesions and separation of the damaged and undamaged strands.

Similarity: Belongs to the WD repeat DDB2/WDR76 family. {ECO:0000305}.

Sequence caution: Sequence=AAI10097.1; Type=Erroneous initiation; Evidence={ECO:0000305}; Sequence=CAK11059.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305}; Sequence=CAK11060.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};

Annotations taken from UniProtKB at the EBI.


Organism:		Danio rerio (Zebrafish) (Brachydanio rerio).
Taxonomy:		Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes; Danionidae; Danioninae; Danio.



Function:		Protein, which is both involved in DNA repair and protein ubiquitination, as part of the UV-DDB complex and DCX (DDB1-CUL4-X-box) complexes, respectively. Core component of the UV-DDB complex (UV- damaged DNA-binding protein complex), a complex that recognizes UV- induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6- 4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also functions as the substrate recognition module for the DCX (DDB2-CUL4-X- box) E3 ubiquitin-protein ligase complex DDB2-CUL4-ROC1 (also known as CUL4-DDB-ROC1 and CUL4-DDB-RBX1). The DDB2-CUL4-ROC1 complex may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV- induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. {ECO:0000250\|UniProtKB:Q92466}.


Pathway:		Protein modification; protein ubiquitination.
Subunit:		Component of the UV-DDB complex which includes ddb1 and ddb2 (PubMed:19109893). Component of a DCX (DDB1-CUL4-X-box) E3 ubiquitin- protein ligase complex that includes cul4a, or cul4b, ddb1, ddb2 and rbx1 (PubMed:22118460). A large number of other DCX complexes may also exist in which an alternate substrate targeting subunit replaces ddb2. These targeting subunits are generally known as DCAF (ddb1- and cul4- associated factor) or CDW (cul4-ddb1-associated WD40-repeat) proteins (By similarity). {ECO:0000250, ECO:0000269\|PubMed:19109893, ECO:0000269\|PubMed:22118460}.
Subcellular location:		Nucleus {ECO:0000250\|UniProtKB:Q92466}. Note=Accumulates at sites of DNA damage following UV irradiation. {ECO:0000250\|UniProtKB:Q92466}.
Domain:		The DWD box is required for interaction with ddb1. {ECO:0000250}.
Domain:		Interblade loops of the WD repeat region mediate most of the interaction with DNA. A hairpin between blades 5 and 6 inserts into DNA minor groove and mediates recognition of lesions and separation of the damaged and undamaged strands.
Similarity:		Belongs to the WD repeat DDB2/WDR76 family. {ECO:0000305}.
Sequence caution:		Sequence=AAI10097.1; Type=Erroneous initiation; Evidence={ECO:0000305}; Sequence=CAK11059.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305}; Sequence=CAK11060.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};