PDBsum entry 4hx3

Hydrolase/hydrolase inhibitor

PDB id: 4hx3

Contents

Protein chains

(+ 0 more) 134 a.a.

(+ 0 more) 113 a.a.

Ligands

GOL ×2

Metals

_ZN ×6

Waters ×275

PDB id:

4hx3

Name:

Hydrolase/hydrolase inhibitor

Title:

Crystal structure of streptomyces caespitosus sermetstatin in complex with s. Caespitosus snapalysin

Structure:

Extracellular small neutral protease. Chain: a, c, e, g, i, k. Fragment: mature protease. Synonym: scnp, snapalysin. Engineered: yes. Neutral proteinase inhibitor scnpi. Chain: b, d, f, h, j, l. Synonym: sermetstatin. Engineered: yes

Source:

Streptomyces caespitosus. Organism_taxid: 53502. Gene: snpa. Expressed in: escherichia coli. Expression_system_taxid: 469008. Gene: scnpi. Expression_system_taxid: 562.

Resolution:

2.70Å R-factor: 0.196 R-free: 0.242

Authors:

S.Trillo-Muyo,S.Martinez-Rodriguez,J.L.Arolas,F.X.Gomis-Ruth

Key ref:

S.Trillo-Muyo et al. Mechanism of action of a janus-Faced single-Domain pr inhibitor simultaneously targeting two peptidase clas. To be published, .

Date:

09-Nov-12 Release date: 05-Dec-12

Protein chains

P56406  (SNPA_STRCS) -  Extracellular small neutral protease from Streptomyces caespitosus

Seq: 132 a.a.

Struc: 134 a.a.*

Protein chains

Q9FDS0  (Q9FDS0_STRCS) -  Neutral proteinase inhibitor ScNPI from Streptomyces caespitosus

Seq: 141 a.a.

Struc: 113 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: Chains A, C, E, G, I, K: E.C.3.4.24.77 - snapalysin.
• Reaction: Hydrolyzes proteins with a preference for Tyr or Phe in the P1' position. Has no action on amino-acid p-nitroanilides.
• Cofactor: Zn(2+)