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PDBsum entry 4fo6
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Transferase, lyase/DNA
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PDB id
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4fo6
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PDB id:
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| Name: |
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Transferase, lyase/DNA
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Title:
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Crystal structure of the pre-catalytic ternary complex of polymerase lambda with a datp analog opposite a templating t and an rcmp at the primer terminus.
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Structure:
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DNA polymerase lambda. Chain: a. Fragment: loop mutant of DNA polymerase lambda. Synonym: pol lambda, DNA polymerase beta-2, pol beta2, DNA polymerase kappa. Engineered: yes. Mutation: yes. 5'-d( Cp Gp Gp Cp Tp Gp Tp Ap Cp Tp G)-3'. Chain: t.
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Source:
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Homo sapiens. Human. Organism_taxid: 9606. Gene: poll. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes.
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Resolution:
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2.01Å
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R-factor:
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0.192
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R-free:
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0.231
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Authors:
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R.A.Gosavi,A.F.Moon,T.A.Kunkel,L.C.Pedersen,K.Bebenek
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Key ref:
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R.A.Gosavi
et al.
(2012).
The catalytic cycle for ribonucleotide incorporation by human DNA Pol λ.
Nucleic Acids Res,
40,
7518-7527.
PubMed id:
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Date:
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20-Jun-12
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Release date:
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11-Jul-12
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Supersedes:
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PROCHECK
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Headers
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References
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Q9UGP5
(DPOLL_HUMAN) -
DNA polymerase lambda from Homo sapiens
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Seq:
Struc:
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575 a.a.
315 a.a.*
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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*
PDB and UniProt seqs differ
at 4 residue positions (black
crosses)
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C-G-G-C-T-G-T-A-C-T-G
11 bases
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C-A-G-T-A-C
6 bases
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G-C-C-G
4 bases
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T-A-C-T-G
5 bases
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C-A-G-T-A
5 bases
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Enzyme class 2:
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E.C.2.7.7.7
- DNA-directed Dna polymerase.
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Reaction:
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DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate
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DNA(n)
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+
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2'-deoxyribonucleoside 5'-triphosphate
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=
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DNA(n+1)
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+
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diphosphate
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Enzyme class 3:
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E.C.4.2.99.-
- ?????
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Note, where more than one E.C. class is given (as above), each may
correspond to a different protein domain or, in the case of polyprotein
precursors, to a different mature protein.
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Molecule diagrams generated from .mol files obtained from the
KEGG ftp site
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Nucleic Acids Res
40:7518-7527
(2012)
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PubMed id:
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The catalytic cycle for ribonucleotide incorporation by human DNA Pol λ.
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R.A.Gosavi,
A.F.Moon,
T.A.Kunkel,
L.C.Pedersen,
K.Bebenek.
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ABSTRACT
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Links
