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PDBsum entry 4ekk

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protein ligands metals Protein-protein interface(s) links
Transferase PDB id
4ekk
Jmol
Contents
Protein chains
319 a.a.
Ligands
GLY-ARG-PRO-ARG-
THR-THR-SER-PHE-
ALA-GLU
×2
ANP ×2
Metals
_MN ×4
Waters ×100
PDB id:
4ekk
Name: Transferase
Title: Akt1 with amp-pnp
Structure: Rac-alpha serine/threonine-protein kinase. Chain: a, b. Fragment: unp residues 144-480. Synonym: protein kinase b, pkb, protein kinase b alpha, pkb proto-oncogenE C-akt, rac-pk-alpha. Engineered: yes. Mutation: yes. Glycogen synthase kinase-3 beta. Chain: c, d.
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: akt1, pkb, rac. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Synthetic: yes. Organism_taxid: 9606
Resolution:
2.80Å     R-factor:   0.220     R-free:   0.280
Authors: W.-I.Wu,G.P.A Vigers,T.H.Morales,B.J.Brandhuber
Key ref: K.Lin et al. (2012). An ATP-site on-off switch that restricts phosphatase accessibility of Akt. Sci Signal, 5, ra37. PubMed id: 22569334
Date:
09-Apr-12     Release date:   23-May-12    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
P31749  (AKT1_HUMAN) -  RAC-alpha serine/threonine-protein kinase
Seq:
Struc:
480 a.a.
319 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 2 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class: E.C.2.7.11.1  - Non-specific serine/threonine protein kinase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: ATP + a protein = ADP + a phosphoprotein
ATP
+
protein
Bound ligand (Het Group name = PRO)
matches with 50.00% similarity
=
ADP
Bound ligand (Het Group name = ANP)
matches with 81.25% similarity
+ phosphoprotein
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     protein phosphorylation   1 term 
  Biochemical function     transferase activity, transferring phosphorus-containing groups     4 terms  

 

 
    reference    
 
 
Sci Signal 5:ra37 (2012)
PubMed id: 22569334  
 
 
An ATP-site on-off switch that restricts phosphatase accessibility of Akt.
K.Lin, J.Lin, W.I.Wu, J.Ballard, B.B.Lee, S.L.Gloor, G.P.Vigers, T.H.Morales, L.S.Friedman, N.Skelton, B.J.Brandhuber.
 
  ABSTRACT  
 
No abstract given.