PDBsum entry 4ebe

Transferase/DNA

PDB id: 4ebe

Contents

Protein chain

376 a.a.

DNA/RNA

Ligands

DTP

GOL ×2

Metals

_CA ×3

Waters ×297

PDB id:

4ebe

Name:

Transferase/DNA

Title:

Conformationally restrained north-methanocarba-2'-deoxyadenosine corrects the error-prone nature of human DNA polymerase iota

Structure:

DNA polymerase iota. Chain: a. Fragment: unp residues 26-445. Synonym: eta2, rad30 homolog b. Engineered: yes. 5'-d(p Ap Gp Gp Ap Cp Cp (Doc))-3'. Chain: p. Engineered: yes. Other_details: 7-mer primer DNA.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: poli, rad30b. Expressed in: escherichia coli. Expression_system_taxid: 469008. Synthetic: yes. Synthetic: yes

Resolution:

2.10Å R-factor: 0.222 R-free: 0.265

Authors:

R.L.Eoff,A.Ketkar,S.Banerjee

Key ref:

A.Ketkar et al. (2012). A nucleotide-analogue-induced gain of function corrects the error-prone nature of human DNA polymerase iota. J Am Chem Soc, 134, 10698-10705. PubMed id: 22632140

Date:

23-Mar-12 Release date: 13-Jun-12

Protein chain

Q9UNA4  (POLI_HUMAN) -  DNA polymerase iota from Homo sapiens

Seq: 740 a.a.

Struc: 376 a.a.

DNA/RNA chains

A-G-G-A-C-C-DOC 7 bases

C-T-G-G-G-T-C-C-T 9 bases

Enzyme reactions

• Enzyme class: E.C.2.7.7.7 - DNA-directed Dna polymerase.
• Reaction: DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

J Am Chem Soc 134:10698-10705 (2012)

PubMed id: 22632140

A nucleotide-analogue-induced gain of function corrects the error-prone nature of human DNA polymerase iota.

A.Ketkar, M.K.Zafar, S.Banerjee, V.E.Marquez, M.Egli, R.L.Eoff.

ABSTRACT

No abstract given.