spacer
spacer

PDBsum entry 4dm9

Go to PDB code: 
protein ligands Protein-protein interface(s) links
Hydrolase, ligase/inhibitor PDB id
4dm9
Jmol
Contents
Protein chain
223 a.a.
Ligands
PHQ-VAL-ALA-GME-
CF0
×2
Waters ×42
PDB id:
4dm9
Name: Hydrolase, ligase/inhibitor
Title: The crystal structure of ubiquitin carboxy-terminal hydrolas (uchl1) bound to a tripeptide fluoromethyl ketone z-vae(ome
Structure: Ubiquitin carboxyl-terminal hydrolase isozyme l1. Chain: a, b. Synonym: uch-l1, neuron cytoplasmic protein 9.5, pgp 9.5, p ubiquitin thioesterase l1. Engineered: yes. Tripeptide fluoromethyl ketone inhibitor z-vae(om chain: x, y. Engineered: yes
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: uchl1. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes
Resolution:
2.35Å     R-factor:   0.200     R-free:   0.250
Authors: C.W.Davies,J.Chaney,G.Korbel,D.Ringe,G.A.Petsko,H.Ploegh,C.D
Key ref: C.W.Davies et al. (2012). The co-crystal structure of ubiquitin carboxy-terminal hydrolase L1 (UCHL1) with a tripeptide fluoromethyl ketone (Z-VAE(OMe)-FMK). Bioorg Med Chem Lett, 22, 3900-3904. PubMed id: 22617491 DOI: 10.1016/j.bmcl.2012.04.124
Date:
07-Feb-12     Release date:   23-May-12    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
P09936  (UCHL1_HUMAN) -  Ubiquitin carboxyl-terminal hydrolase isozyme L1
Seq:
Struc:
223 a.a.
223 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class: E.C.3.4.19.12  - Ubiquitinyl hydrolase 1.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Thiol-dependent hydrolysis of ester, thiolester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     intracellular   12 terms 
  Biological process     response to ischemia   15 terms 
  Biochemical function     protein binding     11 terms  

 

 
DOI no: 10.1016/j.bmcl.2012.04.124 Bioorg Med Chem Lett 22:3900-3904 (2012)
PubMed id: 22617491  
 
 
The co-crystal structure of ubiquitin carboxy-terminal hydrolase L1 (UCHL1) with a tripeptide fluoromethyl ketone (Z-VAE(OMe)-FMK).
C.W.Davies, J.Chaney, G.Korbel, D.Ringe, G.A.Petsko, H.Ploegh, C.Das.
 
  ABSTRACT  
 
No abstract given.