spacer
spacer

PDBsum entry 3zuv
Go to PDB code: 
protein ligands Protein-protein interface(s) links
De novo protein/transferase PDB id
3zuv

 

 

 

 

Loading ...

 
JSmol PyMol  
Contents
Protein chains
359 a.a.
122 a.a.
Ligands
SO4 ×2
Waters ×203
PDB id:
3zuv
Name: De novo protein/transferase
Title: Crystal structure of a designed selected ankyrin repeat protein in complex with the phosphorylated map kinase erk2
Structure: Mitogen-activated protein kinase 1. Chain: a, c. Fragment: residues 3-358. Synonym: map kinase 1, mapk 1, ert1, extracellular signal-regulated kinase 2, erk-2, map kinase isoform p42, p42-mapk, mitogen-activated protein kinase 2, map kinase 2, mapk 2. Engineered: yes. Designed ankyrin repeat protein. Chain: b, d.
Source: Rattus norvegicus. Norway rat. Organism_taxid: 10116. Expressed in: escherichia coli. Expression_system_taxid: 469008. Synthetic construct. Organism_taxid: 32630. Expression_system_taxid: 83333. Expression_system_variant: xl1-blue.
Resolution:
2.72Å     R-factor:   0.178     R-free:   0.230
Authors: L.Kummer,P.R.Mittl,A.Pluckthun
Key ref: L.Kummer et al. (2012). Structural and functional analysis of phosphorylation-specific binders of the kinase ERK from designed ankyrin repeat protein libraries. Proc Natl Acad Sci U S A, 109, E2248. PubMed id: 22843676
Date:
20-Jul-11     Release date:   27-Jun-12    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
P63086  (MK01_RAT) -  Mitogen-activated protein kinase 1 from Rattus norvegicus
Seq:
Struc: 		358 a.a.
359 a.a.*
Protein chains
No UniProt id for this chain
Struc: 122 a.a.
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 3 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class: Chains A, C: E.C.2.7.11.24  - mitogen-activated protein kinase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction:
1. L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H+
2. L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H+
L-seryl-[protein]
 + ATP
 = O-phospho-L-seryl-[protein]
 + ADP
 + H(+)
L-threonyl-[protein]
 + ATP
 = O-phospho-L-threonyl-[protein]
 + ADP
 + H(+)
Molecule diagrams generated from .mol files obtained from the KEGG ftp site

 

 
    reference    
 
 
Proc Natl Acad Sci U S A 109:E2248 (2012)
PubMed id: 22843676  
 
 
Structural and functional analysis of phosphorylation-specific binders of the kinase ERK from designed ankyrin repeat protein libraries.
L.Kummer, P.Parizek, P.Rube, B.Millgramm, A.Prinz, P.R.Mittl, M.Kaufholz, B.Zimmermann, F.W.Herberg, A.Plückthun.
 
  ABSTRACT  
 
No abstract given.

 

 

spacer
spacer