PDBsum entry 3zjy

Translation

PDB id: 3zjy

Contents

Protein chains

169 a.a.

908 a.a.

74 a.a.

825 a.a.

Ligands

GTP ×3

Metals

_MG ×3

PDB id:

3zjy

Name:

Translation

Title:

Crystal structure of importin 13 - rangtp - eif1a complex

Structure:

Gtp-binding nuclear protein ran. Chain: a, d, f. Fragment: residues 1-180. Synonym: androgen receptor-associated protein 24, gtpase ran, ras- like protein tc4, ras-related nuclear protein. Engineered: yes. Mutation: yes. Importin-13. Chain: b, e, g.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 562. Expression_system_taxid: 562

Resolution:

3.60Å R-factor: 0.258 R-free: 0.288

Authors:

M.Gruenwald,D.Lazzaretti,F.Bono

Key ref:

M.Grünwald et al. (2013). Structural basis for the nuclear export activity of Importin13. Embo J, 32, 899-913. PubMed id: 23435562 DOI: 10.1038/emboj.2013.29

Date:

21-Jan-13 Release date: 10-Apr-13

Protein chains

P62826  (RAN_HUMAN) -  GTP-binding nuclear protein Ran from Homo sapiens

Seq: 216 a.a.

Struc: 169 a.a.*

Protein chains

O94829  (IPO13_HUMAN) -  Importin-13 from Homo sapiens

Seq: 963 a.a.

Struc: 908 a.a.

Protein chain

P47813  (IF1AX_HUMAN) -  Eukaryotic translation initiation factor 1A, X-chromosomal from Homo sapiens

Seq: 144 a.a.

Struc: 74 a.a.

Protein chain

O94829  (IPO13_HUMAN) -  Importin-13 from Homo sapiens

Seq: 963 a.a.

Struc: 825 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class 1: Chains A, D, F: E.C.3.6.5.- - ?????
• Enzyme class 2: Chains B, C, E, G: E.C.?

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

DOI no: 10.1038/emboj.2013.29

Embo J 32:899-913 (2013)

PubMed id: 23435562

Structural basis for the nuclear export activity of Importin13.

M.Grünwald, D.Lazzaretti, F.Bono.

ABSTRACT

Importin13 (Imp13) is a bidirectional karyopherin that can mediate both import and export of cargoes. Imp13 recognizes several import cargoes, which include the exon junction complex components Mago-Y14 and the E2 SUMO-conjugating enzyme Ubc9, and one known export cargo, the translation initiation factor 1A (eIF1A). To understand how Imp13 can perform double duty, we determined the 3.6-Å crystal structure of Imp13 in complex with RanGTP and with eIF1A. eIF1A binds at the inner surface of the Imp13 C-terminal arch adjacent and concomitantly to RanGTP illustrating how eIF1A can be exported by Imp13. Moreover, the 3.0-Å structure of Imp13 in its unbound state reveals the existence of an open conformation in the cytoplasm that explains export cargo release and completes the export branch of the Imp13 pathway. Finally, we demonstrate that Imp13 is able to bind and export eIF1A in vivo and that its function is essential.