PDBsum entry 3zia

Hydrolase

PDB id: 3zia

Contents

Protein chains

(+ 0 more) 484 a.a.

(+ 0 more) 470 a.a.

265 a.a.

124 a.a.

58 a.a.

36 a.a.

Ligands

ATP ×2

ADP ×10

EDO ×6

Metals

_MG ×10

Waters ×773

PDB id:

3zia

Name:

Hydrolase

Title:

The structure of f1-atpase from saccharomyces cerevisiae inhibited by its regulatory protein if1

Structure:

Atp synthase subunit alpha, mitochondrial. Chain: a, b, c, k, l, m. Synonym: f1-atpase. Atp synthase subunit beta, mitochondrial. Chain: d, e, f, n, o, p. Synonym: f1-atpase. Atp synthase subunit gamma, mitochondrial. Chain: g, q. Synonym: f-atpase gamma subunit, f1-atpase.

Source:

Saccharomyces cerevisiae. Baker's yeast. Organism_taxid: 4932. Strain: w303-1a. Strain: w303-a1. Expressed in: escherichia coli. Expression_system_taxid: 469008. Expression_system_variant: c41.

Resolution:

2.50Å R-factor: 0.226 R-free: 0.262

Authors:

G.C.Robinson,J.V.Bason,M.G.Montgomery,I.M.Fearnley,D.M.Mueller, A.G.W.Leslie,J.E.Walker

Key ref:

G.C.Robinson et al. (2013). The structure of F₁-ATPase from Saccharomyces cerevisiae inhibited by its regulatory protein IF₁. Open Biol, 3, 120164. PubMed id: 23407639 DOI: 10.1098/rsob.120164

Date:

07-Jan-13 Release date: 13-Feb-13

Protein chains

P07251  (ATPA_YEAST) -  ATP synthase subunit alpha, mitochondrial from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 545 a.a.

Struc: 484 a.a.

Protein chains

P00830  (ATPB_YEAST) -  ATP synthase subunit beta, mitochondrial from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 511 a.a.

Struc: 470 a.a.

Protein chains

P38077  (ATPG_YEAST) -  ATP synthase subunit gamma, mitochondrial from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 311 a.a.

Struc: 265 a.a.

Protein chains

Q12165  (ATPD_YEAST) -  ATP synthase subunit delta, mitochondrial from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 160 a.a.

Struc: 124 a.a.

Protein chains

P21306  (ATP5E_YEAST) -  ATP synthase subunit epsilon, mitochondrial from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 62 a.a.

Struc: 58 a.a.

Protein chains

P01097  (ATIF_YEAST) -  F(1)-ATPase inhibitor IF(1), mitochondrial from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 85 a.a.

Struc: 36 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: Chains D, E, F, N, O, P: E.C.7.1.2.2 - H(+)-transporting two-sector ATPase.
• Reaction: ATP + H2O + 4 H⁺(in) = ADP + phosphate + 5 H⁺(out)

ATP (Bound ligand (Het Group name = ATP) corresponds exactly) + H2O + 4 × H(+)(in) = ADP + phosphate + 5 × H(+)(out)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

DOI no: 10.1098/rsob.120164

Open Biol 3:120164 (2013)

PubMed id: 23407639

The structure of F₁-ATPase from Saccharomyces cerevisiae inhibited by its regulatory protein IF₁.

G.C.Robinson, J.V.Bason, M.G.Montgomery, I.M.Fearnley, D.M.Mueller, A.G.Leslie, J.E.Walker.

ABSTRACT

No abstract given.