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PDBsum entry 3zd7
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Hydrolase/RNA
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PDB id
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3zd7
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References listed in PDB file
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Key reference
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Title
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Defining the functional determinants for RNA surveillance by rig-I.
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Authors
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A.Kohlway,
D.Luo,
D.C.Rawling,
S.C.Ding,
A.M.Pyle.
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Ref.
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Embo Rep, 2013,
14,
772-779.
[DOI no: ]
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PubMed id
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Abstract
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Retinoic acid-inducible gene-I (RIG-I) is an intracellular RNA sensor that
activates the innate immune machinery in response to infection by RNA viruses.
Here, we report the crystal structure of distinct conformations of a RIG-I:dsRNA
complex, which shows that HEL2i-mediated scanning allows RIG-I to sense the
length of RNA targets. To understand the implications of HEL2i scanning for
catalytic activity and signalling by RIG-I, we examined its ATPase activity when
stimulated by duplex RNAs of varying lengths and 5' composition. We identified a
minimal RNA duplex that binds one RIG-I molecule, stimulates robust ATPase
activity, and elicits a RIG-I-mediated interferon response in cells. Our results
reveal that the minimal functional unit of the RIG-I:RNA complex is a monomer
that binds at the terminus of a duplex RNA substrate. This behaviour is markedly
different from the RIG-I paralog melanoma differentiation-associated gene 5
(MDA5), which forms cooperative filaments.
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