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PDBsum entry 3x1u
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Structural protein/DNA
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PDB id
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3x1u
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Contents |
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98 a.a.
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83 a.a.
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105 a.a.
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94 a.a.
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87 a.a.
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References listed in PDB file
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Key reference
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Title
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Structural and functional analyses of nucleosome complexes with mouse histone variants th2a and th2b, Involved in reprogramming.
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Authors
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S.Padavattan,
T.Shinagawa,
K.Hasegawa,
T.Kumasaka,
S.Ishii,
T.Kumarevel.
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Ref.
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Biochem Biophys Res Commun, 2015,
464,
929-935.
[DOI no: ]
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PubMed id
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Abstract
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Histone variants TH2a and TH2b are highly expressed in testes, oocytes and
zygotes. Our recent analysis suggested that these histone variants enhance the
induced generation of pluripotent stem cells (iPSCs) when co-expressed along
with four transcription factors, Oct3/4, Sox2, Klf4 and c-Myc (OSKM), and are
associated with an open chromatin structure [1]. In the present study, we report
the crystal structures of nucleosomes (NCPs) with the mouse histone variants,
TH2a and TH2b. The structures revealed two significant changes, as compared to
the canonical counterparts: fewer histone-DNA contacts and changes in
dimer-dimer interactions between TH2a-TH2a' (L1-loop). In vivo studies with
domain swapping and point mutants of the variants revealed that the residues in
the histone tails and the TH2a-L1 loop are important for reprogramming. Taken
together, our work indicates that the NCP variants with structural modifications
and flexible tails are most likely important for enhanced reprogramming of
functions.
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