 |
PDBsum entry 3wrp
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
DNA binding regulatory protein
|
PDB id
|
|
|
|
3wrp
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
References listed in PDB file
|
|
|
Key reference
|
|
|
Title
|
|
Flexibility of the DNA-Binding domains of trp repressor.
|
|
|
Authors
|
|
C.L.Lawson,
R.G.Zhang,
R.W.Schevitz,
Z.Otwinowski,
A.Joachimiak,
P.B.Sigler.
|
|
|
Ref.
|
|
Proteins, 1988,
3,
18-31.
|
|
|
PubMed id
|
|
|
|
|
|
Abstract
|
|
|
An orthorhombic crystal form of trp repressor (aporepressor plus L-tryptophan
ligand) was solved by molecular replacement, refined to 1.65 A resolution, and
compared to the structure of the repressor in trigonal crystals. Even though
these two crystal forms of repressor were grown under identical conditions, the
refined structures have distinctly different conformations of the DNA-binding
domains. Unlike the repressor/aporepressor structural transition, the
conformational shift is not caused by the binding or loss of the L-tryptophan
ligand. We conclude that while L-tryptophan binding is essential for forming a
specific complex with trp operator DNA, the corepressor ligand does not lock the
repressor into a single conformation that is complementary to the operator. This
flexibility may be required by the various binding modes proposed for trp
repressor in its search for and adherence to its three different operator sites.
|
|
|
Secondary reference #1
|
|
|
Title
|
|
The crystal structure of trp aporepressor at 1.8 angstroms shows how binding tryptophan enhances DNA affinity
|
|
|
Authors
|
|
R.-G.Zhang,
A.Joachimiak,
C.L.Lawson,
R.W.Schevitz,
Z.Otwinowski,
P.B.Sigler.
|
|
|
Ref.
|
|
nature, 1987,
327,
891.
|
|
|
|
Secondary reference #2
|
|
|
Title
|
|
The three-Dimensional structure of trp repressor.
|
|
|
Authors
|
|
R.W.Schevitz,
Z.Otwinowski,
A.Joachimiak,
C.L.Lawson,
P.B.Sigler.
|
|
|
Ref.
|
|
Nature, 1985,
317,
782-786.
|
|
|
PubMed id
|
|
|
|
|
|
|
|
|
Secondary reference #3
|
|
|
Title
|
|
Functional inferences from crystals of escherichia coli trp repressor.
|
|
|
Authors
|
|
A.Joachimiak,
R.W.Schevitz,
R.L.Kelley,
C.Yanofsky,
P.B.Sigler.
|
|
|
Ref.
|
|
J Biol Chem, 1983,
258,
12641-12643.
|
|
|
PubMed id
|
|
|
|
|
|
|
|
|
Secondary reference #4
|
|
|
Title
|
|
Purification and characterization of trp aporepressor.
|
|
|
Authors
|
|
A.Joachimiak,
R.L.Kelley,
R.P.Gunsalus,
C.Yanofsky,
P.B.Sigler.
|
|
|
Ref.
|
|
Proc Natl Acad Sci U S A, 1983,
80,
668-672.
[DOI no: ]
|
|
|
PubMed id
|
|
|
|
|
|
|
|
|
Secondary reference #5
|
|
|
Title
|
|
Nucleotide sequence and expression of escherichia coli trpr, The structural gene for the trp aporepressor.
|
|
|
Authors
|
|
R.P.Gunsalus,
C.Yanofsky.
|
|
|
Ref.
|
|
Proc Natl Acad Sci U S A, 1980,
77,
7117-7121.
[DOI no: ]
|
|
|
PubMed id
|
|
|
|
|
|
|
|
|
|
|
|
|
