PDBsum entry 3wkl

Hydrolase

PDB id: 3wkl

Contents

Protein chain

180 a.a.

PDB id:

3wkl

Name:

Hydrolase

Title:

The periplasmic pdz tandem fragment of the rsep homologue from aquifex aeolicus

Structure:

Putative zinc metalloprotease aq_1964. Chain: a. Fragment: pdz tandem fragment, unp residues 115-292. Engineered: yes

Source:

Aquifex aeolicus. Organism_taxid: 63363. Strain: vf5. Gene: aq_1964. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

2.80Å R-factor: 0.257 R-free: 0.295

Authors:

T.Nogi,S.Tabata,K.Tamura-Kawakami,J.Takagi

Key ref:

Y.Hizukuri et al. (2014). A structure-based model of substrate discrimination by a noncanonical PDZ tandem in the intramembrane-cleaving protease RseP. Structure, 22, 326-336. PubMed id: 24389025 DOI: 10.1016/j.str.2013.12.003

Date:

28-Oct-13 Release date: 18-Dec-13

Protein chain

O67776  (Y1964_AQUAE) -  Putative zinc metalloprotease aq_1964 from Aquifex aeolicus (strain VF5)

Seq: 429 a.a.

Struc: 180 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.3.4.24.- - ?????

DOI no: 10.1016/j.str.2013.12.003

Structure 22:326-336 (2014)

PubMed id: 24389025

A structure-based model of substrate discrimination by a noncanonical PDZ tandem in the intramembrane-cleaving protease RseP.

Y.Hizukuri, T.Oda, S.Tabata, K.Tamura-Kawakami, R.Oi, M.Sato, J.Takagi, Y.Akiyama, T.Nogi.

ABSTRACT

During the extracytoplasmic stress response in Escherichia coli, the intramembrane protease RseP cleaves the anti-σ(E) protein RseA only after the membrane-anchored protease DegS truncates the periplasmic part of RseA that suppresses the action of RseP. Here we analyzed the three-dimensional structure of the two tandemly arranged PSD-95/Dlg/ZO-1 (PDZ) domains (PDZ tandem) present in the periplasmic region of RseP and revealed that the two putative ligand-binding grooves constitute a single pocket-like structure that would lie just above the active center sequestrated within the membrane. Complete removal of the PDZ tandem from RseP led to the intramembrane cleavage of RseA without prior truncation by DegS. Furthermore, mutations expected to destabilize the tertiary structure of the PDZ tandem also caused the deregulation of the sequential cleavage. These observations suggest that the PDZ tandem serves as a size-exclusion filter to accommodate the truncated form of RseA into the active center.