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PDBsum entry 3wkj
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Structural protein/DNA
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PDB id
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3wkj
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Contents |
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97 a.a.
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78 a.a.
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107 a.a.
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93 a.a.
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84 a.a.
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References listed in PDB file
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Key reference
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Title
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Structure of human nucleosome containing the testis-Specific histone variant tsh2b.
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Authors
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T.Urahama,
N.Horikoshi,
A.Osakabe,
H.Tachiwana,
H.Kurumizaka.
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Ref.
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Acta Crystallogr F Struct Biol Commun, 2014,
70,
444-449.
[DOI no: ]
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PubMed id
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Abstract
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The human histone H2B variant TSH2B is highly expressed in testis and may
function in the chromatin transition during spermatogenesis. In the present
study, the crystal structure of the human testis-specific nucleosome containing
TSH2B was determined at 2.8 Å resolution. A local structural difference
between TSH2B and canonical H2B in nucleosomes was detected around the
TSH2B-specific amino-acid residue Ser85. The TSH2B Ser85 residue does not
interact with H4 in the nucleosome, but in the canonical nucleosome the H2B
Asn84 residue (corresponding to the TSH2B Ser85 residue) forms water-mediated
hydrogen bonds with the H4 Arg78 residue. In contrast, the other TSH2B-specific
amino-acid residues did not induce any significant local structural changes in
the TSH2B nucleosome. These findings may provide important information for
understanding how testis-specific histone variants form nucleosomes during
spermatogenesis.
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