 |
PDBsum entry 3wh0
|
|
|
|
References listed in PDB file
|
 |
|
Key reference
|
|
|
Title
|
|
Crowning proteins: modulating the protein surface properties using crown ethers.
|
|
|
Authors
|
|
C.C.Lee,
M.Maestre-Reyna,
K.C.Hsu,
H.C.Wang,
C.I.Liu,
W.Y.Jeng,
L.L.Lin,
R.Wood,
C.C.Chou,
J.M.Yang,
A.H.Wang.
|
|
|
Ref.
|
|
Angew Chem Int Ed Engl, 2014,
53,
13054-13058.
[DOI no: ]
|
|
|
PubMed id
|
|
|
|
|
|
Abstract
|
|
|
Crown ethers are small, cyclic polyethers that have found wide-spread use in
phase-transfer catalysis and, to a certain degree, in protein chemistry. Crown
ethers readily bind metallic and organic cations, including positively charged
amino acid side chains. We elucidated the crystal structures of several
protein-crown ether co-crystals grown in the presence of 18-crown-6. We then
employed biophysical methods and molecular dynamics simulations to compare these
complexes with the corresponding apoproteins and with similar complexes with
ring-shaped low-molecular-weight polyethylene glycols. Our studies show that
crown ethers can modify protein surface behavior dramatically by stabilizing
either intra- or intermolecular interactions. Consequently, we propose that
crown ethers can be used to modulate a wide variety of protein surface
behaviors, such as oligomerization, domain-domain interactions, stabilization in
organic solvents, and crystallization.
|
|
|
|
|
|
|
