PDBsum entry 3w8q

Transferase

PDB id: 3w8q

Contents

Protein chain

318 a.a.

Ligands

AGS

Waters ×57

PDB id:

3w8q

Name:

Transferase

Title:

Structure of the human mitogen-activated protein kinase kinase 1 (mek1)

Structure:

Dual specificity mitogen-activated protein kinase kinase 1. Chain: a. Fragment: kinase domain, unp residues 39-382. Synonym: map kinase kinase 1, mapkk 1, mkk1, erk activator kinase 1, mapk/erk kinase 1, mek 1. Engineered: yes. Mutation: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: map2k1. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.20Å R-factor: 0.201 R-free: 0.268

Authors:

S.Nakae,M.Kitamura,T.Shirai,T.Tada

Key ref:

S.Nakae et al. Structure of the human mitogen-Activated protein kina kinase 1 (mek1) in "dfg-Asp out" conformation. To be published, . PubMed id: 34866601

Date:

20-Mar-13 Release date: 26-Mar-14

Protein chain

Q02750  (MP2K1_HUMAN) -  Dual specificity mitogen-activated protein kinase kinase 1 from Homo sapiens

Seq: 393 a.a.

Struc: 318 a.a.

Enzyme reactions

• Enzyme class: E.C.2.7.12.2 - mitogen-activated protein kinase kinase.
• Reaction:
  1. L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H⁺
  2. L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H⁺
  3. L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H⁺

L-seryl-[protein] (Bound ligand (Het Group name = AGS) matches with 93.75% similarity) + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)

L-threonyl-[protein] (Bound ligand (Het Group name = AGS) matches with 93.75% similarity) + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)

L-tyrosyl-[protein] (Bound ligand (Het Group name = AGS) matches with 93.75% similarity) + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site