PDBsum entry 3w5k

Nuclear protein/metal binding protein

PDB id: 3w5k

Contents

Protein chains

859 a.a.

110 a.a.

Metals

_ZN ×4

Waters ×60

PDB id:

3w5k

Name:

Nuclear protein/metal binding protein

Title:

Crystal structure of snail1 and importin beta complex

Structure:

Importin subunit beta-1. Chain: a. Synonym: importin-90, karyopherin subunit beta-1, nuclear factor p97, pore targeting complex 97 kda subunit, ptac97. Engineered: yes. Zinc finger protein snai1. Chain: b. Synonym: protein snail homolog 1, protein sna. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: kpnb1, ntf97. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: snai1, snah.

Resolution:

2.60Å R-factor: 0.217 R-free: 0.264

Authors:

S.Choi,E.Yamashita,N.Yasuhara,J.Song,S.Y.Son,Y.H.Won,Y.S.Shin, T.Sekimoto,I.Y.Park,Y.Yoneda,S.J.Lee

Key ref:

S.Choi et al. (2014). Structural basis for the selective nuclear import of the C2H2 zinc-finger protein Snail by importin β. Acta Crystallogr D Biol Crystallogr, 70, 1050-1060. PubMed id: 24699649 DOI: 10.1107/S1399004714000972

Date:

30-Jan-13 Release date: 05-Mar-14

Protein chain

Q14974  (IMB1_HUMAN) -  Importin subunit beta-1 from Homo sapiens

Seq: 876 a.a.

Struc: 859 a.a.

Protein chain

O95863  (SNAI1_HUMAN) -  Zinc finger protein SNAI1 from Homo sapiens

Seq: 264 a.a.

Struc: 110 a.a.

DOI no: 10.1107/S1399004714000972

Acta Crystallogr D Biol Crystallogr 70:1050-1060 (2014)

PubMed id: 24699649

Structural basis for the selective nuclear import of the C2H2 zinc-finger protein Snail by importin β.

S.Choi, E.Yamashita, N.Yasuhara, J.Song, S.Y.Son, Y.H.Won, H.R.Hong, Y.S.Shin, T.Sekimoto, I.Y.Park, Y.Yoneda, S.J.Lee.

ABSTRACT

Snail contributes to the epithelial-mesenchymal transition by suppressing E-cadherin in transcription processes. The Snail C2H2-type zinc-finger (ZF) domain functions both as a nuclear localization signal which binds to importin β directly and as a DNA-binding domain. Here, a 2.5 Å resolution structure of four ZF domains of Snail1 complexed with importin β is presented. The X-ray structure reveals that the four ZFs of Snail1 are required for tight binding to importin β in the nuclear import of Snail1. The shape of the ZFs in the X-ray structure is reminiscent of a round snail, where ZF1 represents the head, ZF2-ZF4 the shell, showing a novel interaction mode, and the five C-terminal residues the tail. Although there are many kinds of C2H2-type ZFs which have the same fold as Snail, nuclear import by direct recognition of importin β is observed in a limited number of C2H2-type ZF proteins such as Snail, Wt1, KLF1 and KLF8, which have the common feature of terminating in ZF domains with a short tail of amino acids.