spacer
spacer

PDBsum entry 3w5k

Go to PDB code: 
protein metals Protein-protein interface(s) links
Nuclear protein/metal binding protein PDB id
3w5k
Jmol PyMol
Contents
Protein chains
859 a.a.
110 a.a.
Metals
_ZN ×4
Waters ×60
PDB id:
3w5k
Name: Nuclear protein/metal binding protein
Title: Crystal structure of snail1 and importin beta complex
Structure: Importin subunit beta-1. Chain: a. Synonym: importin-90, karyopherin subunit beta-1, nuclear f pore targeting complex 97 kda subunit, ptac97. Engineered: yes. Zinc finger protein snai1. Chain: b. Synonym: protein snail homolog 1, protein sna. Engineered: yes
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: kpnb1, ntf97. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: snai1, snah.
Resolution:
2.60Å     R-factor:   0.217     R-free:   0.264
Authors: S.Choi,E.Yamashita,N.Yasuhara,J.Song,S.Y.Son,Y.H.Won,Y.S.Shi T.Sekimoto,I.Y.Park,Y.Yoneda,S.J.Lee
Key ref: S.Choi et al. (2014). Structural basis for the selective nuclear import of the C2H2 zinc-finger protein Snail by importin β. Acta Crystallogr D Biol Crystallogr, 70, 1050-1060. PubMed id: 24699649 DOI: 10.1107/S1399004714000972
Date:
30-Jan-13     Release date:   05-Mar-14    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
Q14974  (IMB1_HUMAN) -  Importin subunit beta-1
Seq:
Struc:
 
Seq:
Struc:
876 a.a.
859 a.a.
Protein chain
Pfam   ArchSchema ?
O95863  (SNAI1_HUMAN) -  Zinc finger protein SNAI1
Seq:
Struc:
264 a.a.
110 a.a.
Key:    PfamA domain  PfamB domain  Secondary structure

 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     extracellular region   15 terms 
  Biological process     viral reproduction   43 terms 
  Biochemical function     RNA polymerase II regulatory region sequence-specific DNA binding     17 terms  

 

 
DOI no: 10.1107/S1399004714000972 Acta Crystallogr D Biol Crystallogr 70:1050-1060 (2014)
PubMed id: 24699649  
 
 
Structural basis for the selective nuclear import of the C2H2 zinc-finger protein Snail by importin β.
S.Choi, E.Yamashita, N.Yasuhara, J.Song, S.Y.Son, Y.H.Won, H.R.Hong, Y.S.Shin, T.Sekimoto, I.Y.Park, Y.Yoneda, S.J.Lee.
 
  ABSTRACT  
 
Snail contributes to the epithelial-mesenchymal transition by suppressing E-cadherin in transcription processes. The Snail C2H2-type zinc-finger (ZF) domain functions both as a nuclear localization signal which binds to importin β directly and as a DNA-binding domain. Here, a 2.5 Å resolution structure of four ZF domains of Snail1 complexed with importin β is presented. The X-ray structure reveals that the four ZFs of Snail1 are required for tight binding to importin β in the nuclear import of Snail1. The shape of the ZFs in the X-ray structure is reminiscent of a round snail, where ZF1 represents the head, ZF2-ZF4 the shell, showing a novel interaction mode, and the five C-terminal residues the tail. Although there are many kinds of C2H2-type ZFs which have the same fold as Snail, nuclear import by direct recognition of importin β is observed in a limited number of C2H2-type ZF proteins such as Snail, Wt1, KLF1 and KLF8, which have the common feature of terminating in ZF domains with a short tail of amino acids.
 

 

spacer

spacer