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PDBsum entry 3w56
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Lipid binding protein
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PDB id
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3w56
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References listed in PDB file
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Key reference
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Title
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Defining the interaction of perforin with calcium and the phospholipid membrane.
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Authors
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D.A.Traore,
A.J.Brennan,
R.H.Law,
C.Dogovski,
M.A.Perugini,
N.Lukoyanova,
E.W.Leung,
R.S.Norton,
J.A.Lopez,
K.A.Browne,
H.Yagita,
G.J.Lloyd,
A.Ciccone,
S.Verschoor,
J.A.Trapani,
J.C.Whisstock,
I.Voskoboinik.
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Ref.
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Biochem J, 2013,
456,
323-335.
[DOI no: ]
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PubMed id
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Abstract
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Following its secretion from cytotoxic lymphocytes into the immune synapse,
perforin binds to target cell membranes through its Ca2+-dependent C2 domain.
Membrane-bound perforin then forms pores that allow passage of pro-apoptopic
granzymes into the target cell. In the present study, structural and biochemical
studies reveal that Ca2+ binding triggers a conformational change in the C2
domain that permits four key hydrophobic residues to interact with the plasma
membrane. However, in contrast with previous suggestions, these movements and
membrane binding do not trigger irreversible conformational changes in the
pore-forming MACPF (membrane attack complex/perforin-like) domain, indicating
that subsequent monomer-monomer interactions at the membrane surface are
required for perforin pore formation.
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