PDBsum entry 3vxt

Immune system

PDB id: 3vxt

Contents

Protein chains

199 a.a.

241 a.a.

Waters ×108

PDB id:

3vxt

Name:

Immune system

Title:

T36-5 tcr specific for hla-a24-nef134-10

Structure:

T36-5 tcr alpha chain. Chain: c, a. Engineered: yes. T36-5 tcr beta chain. Chain: d, b. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.50Å R-factor: 0.230 R-free: 0.271

Authors:

A.Shimizu,S.Fukai,A.Yamagata,A.Iwamoto

Key ref:

A.Shimizu et al. (2013). Structure of TCR and antigen complexes at an immunodominant CTL epitope in HIV-1 infection. Sci Rep, 3, 3097. PubMed id: 24192765 DOI: 10.1038/srep03097

Date:

20-Sep-12 Release date: 23-Oct-13

Protein chains

No UniProt id for this chain

Struc: 199 a.a.

Protein chains

No UniProt id for this chain

Struc: 241 a.a.

DOI no: 10.1038/srep03097

Sci Rep 3:3097 (2013)

PubMed id: 24192765

Structure of TCR and antigen complexes at an immunodominant CTL epitope in HIV-1 infection.

A.Shimizu, A.Kawana-Tachikawa, A.Yamagata, C.Han, D.Zhu, Y.Sato, H.Nakamura, T.Koibuchi, J.Carlson, E.Martin, C.J.Brumme, Y.Shi, G.F.Gao, Z.L.Brumme, S.Fukai, A.Iwamoto.

ABSTRACT

We investigated the crystal structure of an HLA-A*2402-restricted CTL epitope in the HIV-1 nef gene (Nef134-10) before (pHLA) or after TCR docking. The wild type epitope and two escape mutants were included in the study. Y135F was an early-appearing major mutation, while F139L was a late-appearing mutation which was selected in the patients without Y135F. F139 was an eminent feature of the Nef134-10 epitope. Wild type-specific TCR was less fit to F139L mutant suggesting that F139L is an escape from the CTL against the wild type epitope. Although Y135F mutation disrupted the hydrogen bond to HLA-A*2402 His70, newly formed hydrogen bond between T138 and His70 kept the conformation of the epitope in the reconstituted pMHC. TCR from Y135F- or dually-specific CTL had unique mode of binding to the mutant epitope. Y135F has been reported as a processing mutant but CTL carrying structurally adequate TCR can be found in the patients.