PDBsum entry 3vut

Transferase

PDB id: 3vut

Contents

Protein chains

203 a.a.

260 a.a.

PDB id:

3vut

Name:

Transferase

Title:

Crystal structures of non-phosphorylated map2k4

Structure:

Dual specificity mitogen-activated protein kinase kinase 4. Chain: a, b. Fragment: unp residues 80-399. Synonym: map kinase kinase 4, mapkk 4, jnk-activating kinase 1, mapk/erk kinase 4, mek 4, sapk/erk kinase 1, sek1, stress-activated protein kinase kinase 1, sapk kinase 1, sapkk-1, sapkk1, c-jun n- terminal kinase kinase 1, jnkk. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: map2k4, jnkk1, mek4, mkk4, prkmk4, sek1, serk1, skk1. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

3.50Å R-factor: 0.335 R-free: 0.407

Authors:

T.Matsumoto,T.Kinoshita,Y.Kirii,T.Tada,A.Yamano

Key ref:

T.Matsumoto et al. (2012). Crystal and solution structures disclose a putative transient state of mitogen-activated protein kinase kinase 4. Biochem Biophys Res Commun, 425, 195-200. PubMed id: 22828509

Date:

05-Jul-12 Release date: 05-Sep-12

Protein chain

P45985  (MP2K4_HUMAN) -  Dual specificity mitogen-activated protein kinase kinase 4 from Homo sapiens

Seq: 399 a.a.

Struc: 203 a.a.

Protein chain

P45985  (MP2K4_HUMAN) -  Dual specificity mitogen-activated protein kinase kinase 4 from Homo sapiens

Seq: 399 a.a.

Struc: 260 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: Chains A, B: E.C.2.7.12.2 - mitogen-activated protein kinase kinase.
• Reaction:
  1. L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H⁺
  2. L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H⁺
  3. L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

Biochem Biophys Res Commun 425:195-200 (2012)

PubMed id: 22828509

Crystal and solution structures disclose a putative transient state of mitogen-activated protein kinase kinase 4.

T.Matsumoto, T.Kinoshita, Y.Kirii, T.Tada, A.Yamano.

ABSTRACT

No abstract given.