PDBsum entry 3vu4

Protein transport

PDB id: 3vu4

Contents

Protein chains

319 a.a.

301 a.a.

Ligands

SO4 ×13

Waters ×138

PDB id:

3vu4

Name:

Protein transport

Title:

Crystal structure of kluyvelomyces marxianus hsv2

Structure:

Kmhsv2. Chain: a, b. Engineered: yes

Source:

Kluyveromyces marxianus. Organism_taxid: 4911. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.60Å R-factor: 0.224 R-free: 0.252

Authors:

Y.Watanabe,N.N.Noda

Key ref:

Y.Watanabe et al. (2012). Structure-based analyses reveal distinct binding sites for Atg2 and phosphoinositides in Atg18. J Biol Chem, 287, 31681-31690. PubMed id: 22851171 DOI: 10.1074/jbc.M112.397570

Date:

15-Jun-12 Release date: 04-Jul-12

Protein chain

J3QW34  (J3QW34_KLUMA) -  KmHsv2 from Kluyveromyces marxianus

Seq: 355 a.a.

Struc: 319 a.a.

Protein chain

J3QW34  (J3QW34_KLUMA) -  KmHsv2 from Kluyveromyces marxianus

Seq: 355 a.a.

Struc: 301 a.a.

DOI no: 10.1074/jbc.M112.397570

J Biol Chem 287:31681-31690 (2012)

PubMed id: 22851171

Structure-based analyses reveal distinct binding sites for Atg2 and phosphoinositides in Atg18.

Y.Watanabe, T.Kobayashi, H.Yamamoto, H.Hoshida, R.Akada, F.Inagaki, Y.Ohsumi, N.N.Noda.

ABSTRACT

Autophagy is an intracellular degradation system by which cytoplasmic materials are enclosed by an autophagosome and delivered to a lysosome/vacuole. Atg18 plays a critical role in autophagosome formation as a complex with Atg2 and phosphatidylinositol 3-phosphate (PtdIns(3)P). However, little is known about the structure of Atg18 and its recognition mode of Atg2 or PtdIns(3)P. Here, we report the crystal structure of Kluyveromyces marxianus Hsv2, an Atg18 paralog, at 2.6 Å resolution. The structure reveals a seven-bladed β-propeller without circular permutation. Mutational analyses of Atg18 based on the K. marxianus Hsv2 structure suggested that Atg18 has two phosphoinositide-binding sites at blades 5 and 6, whereas the Atg2-binding region is located at blade 2. Point mutations in the loops of blade 2 specifically abrogated autophagy without affecting another Atg18 function, the regulation of vacuolar morphology at the vacuolar membrane. This architecture enables Atg18 to form a complex with Atg2 and PtdIns(3)P in parallel, thereby functioning in the formation of autophagosomes at autophagic membranes.