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PDBsum entry 3vtx
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Protein binding
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PDB id
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3vtx
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References listed in PDB file
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Key reference
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Title
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Inter-Phylum structural conservation of the magnetosome-Associated tpr-Containing protein, Mama.
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Authors
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N.Zeytuni,
D.Baran,
G.Davidov,
R.Zarivach.
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Ref.
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J Struct Biol, 2012,
180,
479-487.
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PubMed id
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Abstract
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Magnetotactic bacteria enclose the magnetosome, a unique prokaryotic
sub-cellular organelle that allows the biomineralization of magnetic
nano-crystals. Membrane-coated magnetosomes are arranged into a linear chain
that permits magnetotactic bacteria to navigate geomagnetic fields. Magnetosome
assembly and biomineralization are controlled by conserved
magnetosome-associated proteins, including MamA, a tetra-trico-peptide repeat
(TPR)-containing protein that was shown to coat the magnetosome membrane. In
this study, two MamA structures from Candidatus Magnetobacterium bavaricum
(Mbav) were determined via X-ray crystallography. These structures confirm that
Mbav MamA folds as a sequential TPR protein and shares a high degree of
structural similarity with homologous MamA proteins from Magnetospirillum
species. Furthermore, the two TPR-containing domains of MamA are separated by an
interphylum-conserved region containing a flexible hinge that is involved in
ligand binding and recognition. Finally, substantial differences were found in
the local stabilization of the MamA N-terminal domain as a result of the loss of
an evolutionary conserved salt bridge.
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