PDBsum entry 3vtx

Protein binding

PDB id: 3vtx

Contents

Protein chains

181 a.a.

Ligands

GOL ×2

Waters ×376

PDB id:

3vtx

Name:

Protein binding

Title:

Crystal structure of mama protein

Structure:

Mama. Chain: a, b. Engineered: yes

Source:

Candidatus magnetobacterium bavaricum. Organism_taxid: 29290. Strain: mbav. Gene: mama. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

1.75Å R-factor: 0.164 R-free: 0.200

Authors:

N.Zeytuni,D.Baran,G.Davidov,R.Zarivach

Key ref:

N.Zeytuni et al. (2012). Inter-phylum structural conservation of the magnetosome-associated TPR-containing protein, MamA. J Struct Biol, 180, 479-487. PubMed id: 22917855

Date:

08-Jun-12 Release date: 31-Oct-12

Protein chains

K7N5L8  (K7N5L8_9BACT) -  MamA from Candidatus Magnetobacterium bavaricum

Seq: 184 a.a.

Struc: 181 a.a.

J Struct Biol 180:479-487 (2012)

PubMed id: 22917855

Inter-phylum structural conservation of the magnetosome-associated TPR-containing protein, MamA.

N.Zeytuni, D.Baran, G.Davidov, R.Zarivach.

ABSTRACT

Magnetotactic bacteria enclose the magnetosome, a unique prokaryotic sub-cellular organelle that allows the biomineralization of magnetic nano-crystals. Membrane-coated magnetosomes are arranged into a linear chain that permits magnetotactic bacteria to navigate geomagnetic fields. Magnetosome assembly and biomineralization are controlled by conserved magnetosome-associated proteins, including MamA, a tetra-trico-peptide repeat (TPR)-containing protein that was shown to coat the magnetosome membrane. In this study, two MamA structures from Candidatus Magnetobacterium bavaricum (Mbav) were determined via X-ray crystallography. These structures confirm that Mbav MamA folds as a sequential TPR protein and shares a high degree of structural similarity with homologous MamA proteins from Magnetospirillum species. Furthermore, the two TPR-containing domains of MamA are separated by an interphylum-conserved region containing a flexible hinge that is involved in ligand binding and recognition. Finally, substantial differences were found in the local stabilization of the MamA N-terminal domain as a result of the loss of an evolutionary conserved salt bridge.