UniProt functional annotation for Q9JIY2



	UniProt functional annotation for Q9JIY2

UniProt code: Q9JIY2.

Organism: Mus musculus (Mouse).

Taxonomy: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus.

Function: E3 ubiquitin-protein ligase that mediates ubiquitination of several tyrosine-phosphorylated Src substrates, including CDH1, CTTN and DOK1 (PubMed:11836526, PubMed:22252131). Targets CDH1 for endocytosis and degradation (PubMed:11836526). Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing (PubMed:29535189, PubMed:29547716). Its function in the WMM complex is unknown (PubMed:29535189, PubMed:29547716). {ECO:0000269|PubMed:11836526, ECO:0000269|PubMed:22252131, ECO:0000269|PubMed:29535189, ECO:0000269|PubMed:29547716}.

Catalytic activity: Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L- cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.; EC=2.3.2.27; Evidence={ECO:0000269|PubMed:11836526};

Pathway: Protein modification; protein ubiquitination. {ECO:0000269|PubMed:11836526}.

Subunit: Homodimer (PubMed:22252131). Interacts with tyrosine- phosphorylated SRC substrates (PubMed:11836526, PubMed:22252131). Component of the WMM complex, a N6-methyltransferase complex composed of a catalytic subcomplex, named MAC, and of an associated subcomplex, named MACOM (PubMed:29535189, PubMed:29547716). The MAC subcomplex is composed of METTL3 and METTL14 (PubMed:29535189, PubMed:29547716). The MACOM subcomplex is composed of WTAP, ZC3H13, CBLL1/HAKAI, VIRMA, and, in some cases of RBM15 (RBM15 or RBM15B) (PubMed:29535189, PubMed:29547716). Also component of a MACOM-like complex, named WTAP complex, composed of WTAP, ZC3H13, CBLL1, VIRMA, RBM15, BCLAF1 and THRAP3 (By similarity). {ECO:0000250|UniProtKB:Q75N03, ECO:0000269|PubMed:11836526, ECO:0000269|PubMed:22252131, ECO:0000269|PubMed:29535189, ECO:0000269|PubMed:29547716}.

Subcellular location: Nucleus speckle {ECO:0000250|UniProtKB:Q75N03}. Nucleus, nucleoplasm {ECO:0000269|PubMed:29547716}. Cytoplasm {ECO:0000269|PubMed:29547716}. Note=Mainly nuclear with some fraction located in the cytoplasm (PubMed:29547716). ZC3H13 is required to anchor component of the MACOM subcomplex, such as VIRMA, in the nucleus (PubMed:29547716). {ECO:0000269|PubMed:29547716}.

Tissue specificity: Detected in heart, brain, spleen, lung, liver, skeletal muscle, kidney and testis. {ECO:0000269|PubMed:11836526}.

Domain: The HYB domain forms a phosphotyrosine-binding pocket upon dimerization, and mediates as well the recognition of its flanking acidic amino acids. {ECO:0000269|PubMed:22252131}.

Ptm: Phosphorylated on tyrosine residues. {ECO:0000269|PubMed:11836526}.

Similarity: Belongs to the Hakai family. {ECO:0000305}.

Sequence caution: Sequence=BAE38522.1; Type=Frameshift; Evidence={ECO:0000305};

Annotations taken from UniProtKB at the EBI.


Organism:		Mus musculus (Mouse).
Taxonomy:		Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus.



Function:		E3 ubiquitin-protein ligase that mediates ubiquitination of several tyrosine-phosphorylated Src substrates, including CDH1, CTTN and DOK1 (PubMed:11836526, PubMed:22252131). Targets CDH1 for endocytosis and degradation (PubMed:11836526). Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing (PubMed:29535189, PubMed:29547716). Its function in the WMM complex is unknown (PubMed:29535189, PubMed:29547716). {ECO:0000269\|PubMed:11836526, ECO:0000269\|PubMed:22252131, ECO:0000269\|PubMed:29535189, ECO:0000269\|PubMed:29547716}.


Catalytic activity:		Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L- cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.; EC=2.3.2.27; Evidence={ECO:0000269\|PubMed:11836526};
Pathway:		Protein modification; protein ubiquitination. {ECO:0000269\|PubMed:11836526}.
Subunit:		Homodimer (PubMed:22252131). Interacts with tyrosine- phosphorylated SRC substrates (PubMed:11836526, PubMed:22252131). Component of the WMM complex, a N6-methyltransferase complex composed of a catalytic subcomplex, named MAC, and of an associated subcomplex, named MACOM (PubMed:29535189, PubMed:29547716). The MAC subcomplex is composed of METTL3 and METTL14 (PubMed:29535189, PubMed:29547716). The MACOM subcomplex is composed of WTAP, ZC3H13, CBLL1/HAKAI, VIRMA, and, in some cases of RBM15 (RBM15 or RBM15B) (PubMed:29535189, PubMed:29547716). Also component of a MACOM-like complex, named WTAP complex, composed of WTAP, ZC3H13, CBLL1, VIRMA, RBM15, BCLAF1 and THRAP3 (By similarity). {ECO:0000250\|UniProtKB:Q75N03, ECO:0000269\|PubMed:11836526, ECO:0000269\|PubMed:22252131, ECO:0000269\|PubMed:29535189, ECO:0000269\|PubMed:29547716}.
Subcellular location:		Nucleus speckle {ECO:0000250\|UniProtKB:Q75N03}. Nucleus, nucleoplasm {ECO:0000269\|PubMed:29547716}. Cytoplasm {ECO:0000269\|PubMed:29547716}. Note=Mainly nuclear with some fraction located in the cytoplasm (PubMed:29547716). ZC3H13 is required to anchor component of the MACOM subcomplex, such as VIRMA, in the nucleus (PubMed:29547716). {ECO:0000269\|PubMed:29547716}.
Tissue specificity:		Detected in heart, brain, spleen, lung, liver, skeletal muscle, kidney and testis. {ECO:0000269\|PubMed:11836526}.
Domain:		The HYB domain forms a phosphotyrosine-binding pocket upon dimerization, and mediates as well the recognition of its flanking acidic amino acids. {ECO:0000269\|PubMed:22252131}.
Ptm:		Phosphorylated on tyrosine residues. {ECO:0000269\|PubMed:11836526}.
Similarity:		Belongs to the Hakai family. {ECO:0000305}.
Sequence caution:		Sequence=BAE38522.1; Type=Frameshift; Evidence={ECO:0000305};