PDBsum entry 3v7t

Hydrolase/hydrolase inhibitor

PDB id: 3v7t

Contents

Protein chain

242 a.a.

Ligands

0GX ×4

CO3 ×2

Waters ×588

PDB id:

3v7t

Name:

Hydrolase/hydrolase inhibitor

Title:

Crystal structure of human beta-tryptase complexed with a synthetic inhibitor with a tropanylamide scaffold

Structure:

Tpsb2 protein. Chain: a, b, c, d. Fragment: unp residues 38-282. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: tpsb2. Expressed in: komagataella pastoris. Expression_system_taxid: 4922

Resolution:

2.09Å R-factor: 0.210 R-free: 0.234

Authors:

Y.Zhang,C.Colonna,N.Michot

Key ref:

G.Liang et al. (2012). A β-tryptase inhibitor with a tropanylamide scaffold to improve in vitro stability and to lower hERG channel binding affinity. Bioorg Med Chem Lett, 22, 1606-1610. PubMed id: 22264487

Date:

22-Dec-11 Release date: 14-Mar-12

Protein chains

P20231  (TRYB2_HUMAN) -  Tryptase beta-2 from Homo sapiens

Seq: 275 a.a.

Struc: 242 a.a.*

* PDB and UniProt seqs differ at 4 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.4.21.59 - tryptase.
• Reaction: Preferential cleavage: Arg-|-, Lys-|-, but with more restricted specificity than trypsin.

Bioorg Med Chem Lett 22:1606-1610 (2012)

PubMed id: 22264487

A β-tryptase inhibitor with a tropanylamide scaffold to improve in vitro stability and to lower hERG channel binding affinity.

G.Liang, Y.M.Choi-Sledeski, P.Shum, X.Chen, G.B.Poli, V.Kumar, A.Minnich, Q.Wang, J.Tsay, K.Sides, J.Kang, Y.Zhang.

ABSTRACT

No abstract given.