PDBsum entry 3v61

Protein binding/DNA binding protein

PDB id

3v61

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Contents

			Protein chains

					77 a.a.


					254 a.a.

			Ligands

					NEQ ×2

			Metals

					_BA ×13

			Waters ×90

PDB id:

3v61

Links

Name:

Protein binding/DNA binding protein

Title:

Structure of s. Cerevisiae pcna conjugated to sumo on lysine 164

Structure:

Ubiquitin-like protein smt3. Chain: a. Fragment: unp residues 20-98. Engineered: yes. Proliferating cell nuclear antigen. Chain: b. Synonym: pcna. Engineered: yes. Mutation: yes

Source:

Saccharomyces cerevisiae. Baker's yeast. Organism_taxid: 559292. Strain: w3031a. Gene: d9719.15, smt3, ydr510w. Expressed in: escherichia coli. Expression_system_taxid: 469008. Gene: pol30, ybr0811, ybr088c.

Resolution:

2.80Å

R-factor:

0.216

R-free:

0.254

Authors:

A.A.Armstrong,F.Mohideen,C.D.Lima

Key ref:

A.A.Armstrong et al. (2012). Recognition of SUMO-modified PCNA requires tandem receptor motifs in Srs2. Nature, 483, 59-63. PubMed id: 22382979

Date:

18-Dec-11

Release date:

29-Feb-12

PROCHECK

	Headers

	References

Protein chain

Q12306 (SMT3_YEAST) - Ubiquitin-like protein SMT3 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: Struc:					101 a.a. 77 a.a.

Protein chain

P15873 (PCNA_YEAST) - Proliferating cell nuclear antigen from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: Struc:					258 a.a. 254 a.a.*

Key:

PfamA domain

Secondary structure

CATH domain

* PDB and UniProt seqs differ at 1 residue position (black cross)

	Nature 483:59-63 (2012)
PubMed id: 22382979

Recognition of SUMO-modified PCNA requires tandem receptor motifs in Srs2.
A.A.Armstrong, F.Mohideen, C.D.Lima.

ABSTRACT

Ubiquitin (Ub) and ubiquitin-like (Ubl) modifiers such as SUMO (also known as Smt3 in Saccharomyces cerevisiae) mediate signal transduction through post-translational modification of substrate proteins in pathways that control differentiation, apoptosis and the cell cycle, and responses to stress such as the DNA damage response. In yeast, the proliferating cell nuclear antigen PCNA (also known as Pol30) is modified by ubiquitin in response to DNA damage and by SUMO during S phase. Whereas Ub-PCNA can signal for recruitment of translesion DNA polymerases, SUMO-PCNA signals for recruitment of the anti-recombinogenic DNA helicase Srs2. It remains unclear how receptors such as Srs2 specifically recognize substrates after conjugation to Ub and Ubls. Here we show, through structural, biochemical and functional studies, that the Srs2 carboxy-terminal domain harbours tandem receptor motifs that interact independently with PCNA and SUMO and that both motifs are required to recognize SUMO-PCNA specifically. The mechanism presented is pertinent to understanding how other receptors specifically recognize Ub- and Ubl-modified substrates to facilitate signal transduction.

Literature references that cite this PDB file's key reference

PubMed id

Reference

23202584

C.Otomo, Z.Metlagel, G.Takaesu, and T.Otomo (2013).
Structure of the human ATG12~ATG5 conjugate required for LC3 lipidation in autophagy.

Nat Struct Mol Biol, 20, 59-66.

PDB codes:

4gdk 4gdl

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.